Human CYP1B1 protein (Recombinant) (N-GST) (STJP015393)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CYP1B1-N-GST protein was developed from e.coli for the region N-GST. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for further information. |
Target Information
| Gene Symbol | CYP1B1 |
| Gene ID | 1545 |
| Uniprot ID | CP1B1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Leu224-Asp492 |
Additional Info
| Function | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Exhibits catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2- and 4-hydroxy E1 and E2. Displays a predominant hydroxylase activity toward E2 at the C-4 position. Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites. May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8,9-, 11,12-, and 14,15- EpETrE, that function as lipid mediators in the vascular system. Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs). This activity is independent of cytochrome P450 reductase, NADPH, and O2. Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products. Plays an important role in retinal vascular development. Under hyperoxic O2 conditions, promotes retinal angiogenesis and capillary morphogenesis, likely by metabolizing the oxygenated products generated during the oxidative stress. Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression. |
| Protein Name | Cytochrome P450 1b1Cypib1Hydroperoxy Icosatetraenoate Dehydratase |
| Database Links | Reactome: R-HSA-211976Reactome: R-HSA-2142670Reactome: R-HSA-2142816Reactome: R-HSA-5579000 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinMicrosome MembraneMitochondrionLocated Primarily In Endoplasmic ReticulumUpon Treatment With 2378-Tetrachlorodibenzo-P-Dioxin (Tcdd)Cyp1b1 Is Also Targeted To Mitochondria |
| Alternative Protein Names | Cytochrome P450 1b1 proteinCypib1 proteinHydroperoxy Icosatetraenoate Dehydratase proteinCYP1B1 protein |
Information sourced from Uniprot.org