| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human CXCR4-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Gene Symbol: |
CXCR4 |
| Gene ID: |
7852 |
| Uniprot ID: |
CXCR4_HUMAN |
| Immunogen Region: |
MET1-SER46 |
| Immunogen: |
Optimized DNA sequence encoding extracellular domain of Human CXCR4 MET1-SER46) including a N-terminal IgG1 Fc tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. |
| Post Translational Modifications | Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation. Ubiquitinated after ligand binding, leading to its degradation. Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S. Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization. Tyr-7 and Tyr-12 are sulfated in a sequential manner after Tyr-21 is almost fully sulfated, with the binding affinity for CXCL12/SDF-1alpha increasing with the number of sulfotyrosines present. Sulfotyrosines Tyr-7 and Tyr-12 occupy clefts on opposing CXCL12 subunits, thus bridging the CXCL12 dimer interface and promoting CXCL12 dimerization. O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity. |
| Function | Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g. during wound healing. Acts as a receptor for extracellular ubiquitin.leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) for human immunodeficiency virus-1/HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. |
| Protein Name | C-X-C Chemokine Receptor Type 4Cxc-R4Cxcr-4Fb22FusinHm89Lcr1Leukocyte-Derived Seven Transmembrane Domain ReceptorLestrLipopolysaccharide-Associated Protein 3Lap-3Lps-Associated Protein 3NpyrlStromal Cell-Derived Factor 1 ReceptorSdf-1 ReceptorCd Antigen Cd184 |
| Database Links | Reactome: R-HSA-173107Reactome: R-HSA-376176Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-9823730Reactome: R-HSA-9827857 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell JunctionEarly EndosomeLate EndosomeLysosomeIn Unstimulated CellsDiffuse Pattern On Plasma MembraneOn Agonist StimulationColocalizes With Itch At The Plasma Membrane Where It Becomes UbiquitinatedIn The Presence Of AntigenDistributes To The Immunological Synapse Forming At The T-Cell-Apc Contact AreaWhere It Localizes At The Peripheral And Distal Supramolecular Activation Cluster (Smac) |
| Alternative Protein Names | C-X-C Chemokine Receptor Type 4 proteinCxc-R4 proteinCxcr-4 proteinFb22 proteinFusin proteinHm89 proteinLcr1 proteinLeukocyte-Derived Seven Transmembrane Domain Receptor proteinLestr proteinLipopolysaccharide-Associated Protein 3 proteinLap-3 proteinLps-Associated Protein 3 proteinNpyrl proteinStromal Cell-Derived Factor 1 Receptor proteinSdf-1 Receptor proteinCd Antigen Cd184 proteinCXCR4 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
