Human CXCL10/IP-10 protein (Recombinant) (C-His) (STJP020078)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Human CXCL10/IP-10 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val22-Pro98) of Human CXCL10/IP-10 Protein (Accession #NP_001556.2) fused with His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human CXCL10/IP-10-C-His protein was developed in e.coli using the region Val22-Pro98. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CXCL10 |
| Gene ID | 3627 |
| Uniprot ID | CXL10_HUMAN |
| Immunogen | Recombinant Human CXCL10/IP-10 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val22-Pro98) of Human CXCL10/IP-10 Protein (Accession #NP_001556.2) fused with His tag at the C-terminus. |
| Immunogen Region | Val22-Pro98 |
| Immunogen Sequence | VPLSRTVRCTCISISNQPVN PRSLEKLEIIPASQFCPRVE IIATMKKKGEKRCLNPESKA IKNLLKAVSKERSKRSP |
Additional Info
| Tissue Specificity | Mainly secreted by monocytes, endothelial cells as well as fibroblasts. Expressed by epithelial cells in thymus. Microglial cells produce CXCL10 in response to viral stimulation. |
| Post Translational Modifications | Several proteases can mediate post-secretion cleavages. DPP4 cleaves CXCL10 on its N-terminal 2 amino acids leading to an antagonist form of CXCL10. This dominant negative form is capable of binding CXCR3 but does not induce signaling. MMP9 cleaves 9 amino acids instead. |
| Function | Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization. |
| Protein Name | C-X-C Motif Chemokine 1010 Kda Interferon Gamma-Induced ProteinGamma-Ip10Ip-10Small-Inducible Cytokine B10 Cleaved Into - Cxcl10(1-73 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-6783783 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | C-X-C Motif Chemokine 10 protein10 Kda Interferon Gamma-Induced Protein proteinGamma-Ip10 proteinIp-10 proteinSmall-Inducible Cytokine B10 Cleaved Into - Cxcl10(1-73 proteinCXCL10 proteinINP10 proteinSCYB10 protein |
Information sourced from Uniprot.org