Human CX3CL1 (Fractalkine) Antibody Pair (STJAP00193)
General Information
| Short Description | This CX3CL1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | CX3CL1 |
| Gene ID | 6376 |
| Uniprot ID | X3CL1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human CX3CL1 protein.Detection Immunogen: Recombinant Human CX3CL1 protein. |
Additional Info
| Post Translational Modifications | A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. Processed fractalkine: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. Fractalkine: The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. (Microbial infection) Mediates the cytoadherence of erythrocytes infected with parasite P.falciparum (strain 3D7) with endothelial cells by interacting with P.falciparum CBP1 and CBP2 expressed at the surface of erythrocytes. The adhesion prevents the elimination of infected erythrocytes by the spleen (Probable). |
| Protein Name | FractalkineC-X3-C Motif Chemokine 1Cx3c Membrane-Anchored ChemokineNeurotactinSmall-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinProcessed Fractalkine: Secreted |
| Alternative Antibody Pair Names | Fractalkine antibody pairC-X3-C Motif Chemokine 1 antibody pairCx3c Membrane-Anchored Chemokine antibody pairNeurotactin antibody pairSmall-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine antibody pairCX3CL1 antibody pairFKN antibody pairNTT antibody pairSCYD1 antibody pairA-152E5.2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human CX3CL1 (Fractalkine) Antibody Pair (STJAP00193)
This antibody pair detects human CX3CL1 (Fractalkine), corresponding to UniProt entry P78423. Reactivity is confirmed for human samples only.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated. Both are mouse monoclonal antibodies supplied at 0.5 mg/mL, formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human CX3CL1 protein. Both antibodies were purified using Protein A or G affinity chromatography.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.