Human CRACR2A protein (Recombinant) (N-His) (STJP008993)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CRACR2A-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | CRACR2A |
| Gene ID | 84766 |
| Uniprot ID | EFC4B_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp543-Gly731 |
Additional Info
| Function | Isoform 1: Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex. Isoform 2: Rab GTPase that mediates the trafficking of Weibel-Palade bodies (WPBs) to microtubule organizing center (MTOC) in endothelial cells in response to acute inflammatory stimuli. During histamine (but not thrombin) stimulation of endothelial cells, the dynein-bound form induces retrograde transport of a subset of WPBs along microtubules to the MTOC in a Ca(2+)-independent manner and its GTPase activity is essential for this function. Ca(2+)-regulated dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules and regulates endosomal trafficking of CD47. Acts as an intracellular signaling module bridging two important T-cell receptor (TCR) signaling pathways, Ca(2+)-NFAT and JNK, to affect T-cell activation. In resting T-cells, is predominantly localized near TGN network in a GTP-bound form, upon TCR stimulation, localizes at the immunological synapse via interaction with VAV1 to activate downstream Ca(2+)-NFAT and JNK signaling pathways. Plays a role in T-helper 1 (Th1) cell differentiation and T-helper 17 (Th17) cell effector function. Plays a role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. |
| Protein Name | Ef-Hand Calcium-Binding Domain-Containing Protein 4bCalcium Release-Activated Calcium Channel Regulator 2aCrac Channel Regulator 2aCalcium Release-Activated Channel Regulator 2aRas-Related Protein Rab-46 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: CytoplasmIsoform 2: CytoplasmCytoplasmCytoskeletonMicrotubule Organizing CenterCell MembraneGolgi Apparatus MembraneGolgi ApparatusTrans-Golgi Network MembraneVesicleT-Cell Activation-Induced Elevation Of Intracellular Of Ca(2+) Stimulates Its Transport Toward The Microtubule Organizing Center (Mtoc)Histamine Stimulation Induces A Trafficking To The Mtoc In A Gtp-Binding-Dependent But Ca(2+)-Binding-Independent MannerLocalizes To Golgi Membrane In Resting T-Cells And Upon Its Interaction With Vav1Is Translocated From The Golgi Membrane To The Immunological Synapse Via Subsynaptic VesiclesIts Localization In The Golgi Membrane Requires Isoprenylation And Gtp-Binding |
| Alternative Protein Names | Ef-Hand Calcium-Binding Domain-Containing Protein 4b proteinCalcium Release-Activated Calcium Channel Regulator 2a proteinCrac Channel Regulator 2a proteinCalcium Release-Activated Channel Regulator 2a proteinRas-Related Protein Rab-46 proteinCRACR2A proteinEFCAB4B proteinRAB46 protein |
Information sourced from Uniprot.org