Human CP (Ceruloplasmin) Sandwich ELISA Kit (STJE0004038)

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	CP
Gene ID:	1356
Uniprot ID:	CERU_HUMAN
Specificity:	This kit recognizes Human CP in samples. No significant cross-reactivity or interference between Human CP and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Multifunctional blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Copper ions provide a large number of enzymatic activites. Oxidizes highly toxic ferrous ions to the ferric state for further incorporation onto apo-transferrins, catalyzes Cu(+) oxidation and promotes the oxidation of biogenic amines such as norepinephrin and serotonin. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. Has glutathione peroxidase-like activity, can remove both hydrogen peroxide and lipid hydroperoxide in the presence of thiols. Also shows NO-oxidase and NO2 synthase activities that determine endocrine NO homeostasis.
Protein Name	Ceruloplasmin Cuproxidase Ceruloplasmin Ferroxidase Ceruloplasmin Glutathione Peroxidase Ceruloplasmin Glutathione-Dependent Peroxiredoxin Ceruloplasmin
Database Links	Reactome: R-HSA-381426 Reactome: R-HSA-425410 Reactome: R-HSA-5619049 Reactome: R-HSA-5619060 Reactome: R-HSA-8957275 Reactome: R-HSA-917937
Cellular Localisation	Secreted Colocalizes With Gcp1 In Secretory Intracellular Compartments
Alternative ELISA Names	Ceruloplasmin ELISA kit Cuproxidase Ceruloplasmin ELISA kit Ferroxidase Ceruloplasmin ELISA kit Glutathione Peroxidase Ceruloplasmin ELISA kit Glutathione-Dependent Peroxiredoxin Ceruloplasmin ELISA kit CP ELISA kit
Specificity	This kit recognizes Human CP in samples. No significant cross-reactivity or interference between Human CP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-101	95
EDTA plasma (n=8)	90-102	96
Cell culture media (n=8)	85-100	93

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (μg/mL)	2.02	5.90	21.85	2.23	5.36	20.31
Standard deviation	0.12	0.29	1.11	0.11	0.26	0.87
CV (%)	5.94	4.92	5.08	4.93	4.85	4.28

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance