| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description : | This human COL7A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | COL7A1 |
| Gene ID: | 1294 |
| Uniprot ID: | CO7A1_HUMAN |
| Specificity: | This kit recognizes Human COL7 in samples. No significant cross-reactivity or interference between Human COL7 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. |
| Function | Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. |
| Protein Name | Collagen Alpha-1(Vii Chain Long-Chain Collagen Lc Collagen |
| Database Links | Reactome: R-HSA-1442490 Reactome: R-HSA-1474244 Reactome: R-HSA-1650814 Reactome: R-HSA-2022090 Reactome: R-HSA-204005 Reactome: R-HSA-216083 Reactome: R-HSA-2214320 Reactome: R-HSA-3000157 Reactome: R-HSA-5694530 Reactome: R-HSA-8948216 |
| Cellular Localisation | Secreted Extracellular Space Extracellular Matrix Basement Membrane |
| Alternative CLIA Names | Collagen Alpha-1(Vii Chain CLIA kit Long-Chain Collagen CLIA kit Lc Collagen CLIA kit COL7A1 CLIA kit |
| Specificity | This kit recognizes Human COL7 in samples. No significant cross-reactivity or interference between Human COL7 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-101 | 92 |
| EDTA plasma(n=8) | 98-114 | 105 |
| Cell culture media(n=8) | 85-101 | 92 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 99.67 | 298.14 | 708.17 | 105.25 | 327.56 | 674.86 |
| Standard deviation | 10.81 | 21.14 | 68.48 | 12.86 | 29.68 | 51.36 |
| CV (%) | 10.85 | 7.09 | 9.67 | 12.22 | 9.06 | 7.61 |