| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This human COL17A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
COL17A1 |
| Gene ID: |
1308 |
| Uniprot ID: |
COHA1_HUMAN |
| Specificity: |
This kit recognizes Human COL17 in samples. No significant cross-reactivity or interference between Human COL17 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in skin. In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level). Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves. |
| Post Translational Modifications | The intracellular/endo domain is disulfide-linked. Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosphorylation at Ser-544. |
| Function | May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies. |
| Protein Name | Collagen Alpha-1(Xvii Chain180 Kda Bullous Pemphigoid Antigen 2Bullous Pemphigoid Antigen 2 Cleaved Into - 120 Kda Linear Iga Disease Antigen120 Kda Linear Iga Dermatosis AntigenLinear Iga Disease Antigen 1Lad-1 - 97 Kda Linear Iga Disease Antigen97 Kda Linear Iga Bullous Dermatosis Antigen97 Kda Lad Antigen97-LadLinear Iga Bullous Disease Antigen Of 97 KdaLabd97 |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1650814Reactome: R-HSA-198933Reactome: R-HSA-2022090Reactome: R-HSA-446107Reactome: R-HSA-8948216 |
| Cellular Localisation | Cell JunctionHemidesmosomeMembraneSingle-Pass Type Ii Membrane ProteinLocalized Along The Plasma Membrane Of The Hemidesmosome120 Kda Linear Iga Disease Antigen: SecretedExtracellular SpaceExtracellular MatrixBasement MembraneExclusively Localized To Anchoring FilamentsLocalized To The Epidermal Side Of Split Skin97 Kda Linear Iga Disease Antigen: SecretedLocalized In The Lamina Lucida Beneath The Hemidesmosomes |
| Alternative CLIA Names | Collagen Alpha-1(Xvii Chain CLIA kit180 Kda Bullous Pemphigoid Antigen 2 CLIA kitBullous Pemphigoid Antigen 2 Cleaved Into - 120 Kda Linear Iga Disease Antigen CLIA kit120 Kda Linear Iga Dermatosis Antigen CLIA kitLinear Iga Disease Antigen 1 CLIA kitLad-1 - 97 Kda Linear Iga Disease Antigen CLIA kit97 Kda Linear Iga Bullous Dermatosis Antigen CLIA kit97 Kda Lad Antigen CLIA kit97-Lad CLIA kitLinear Iga Bullous Disease Antigen Of 97 Kda CLIA kitLabd97 CLIA kitCOL17A1 CLIA kitBP180 CLIA kitBPAG2 CLIA kit |
| Specificity | This kit recognizes Human COL17 in samples. No significant cross-reactivity or interference between Human COL17 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-102 | 95 |
| EDTA plasma(n=8) | 93-107 | 99 |
| Cell culture media(n=8) | 96-111 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.01 | 4.03 | 17.44 | 2.10 | 4.14 | 15.96 |
| Standard deviation | 0.22 | 0.29 | 1.35 | 0.26 | 0.30 | 1.21 |
| CV (%) | 10.95 | 7.20 | 7.74 | 12.38 | 7.25 | 7.58 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
