This COL17A1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications
ELISA
Reactivity
Human
Sensitivity
1.88ng/mL
Detection Limit
3.13~200ng/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Human COL17 in samples. No significant cross-reactivity or interference between Human COL17 and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Post Translational Modifications
The intracellular/endo domain is disulfide-linked. Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosphorylation at Ser-544.
Function
May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies.
Protein Name
Collagen Alpha-1(Xvii Chain180 Kda Bullous Pemphigoid Antigen 2Bullous Pemphigoid Antigen 2 Cleaved Into - 120 Kda Linear Iga Disease Antigen120 Kda Linear Iga Dermatosis AntigenLinear Iga Disease Antigen 1Lad-1 - 97 Kda Linear Iga Disease Antigen97 Kda Linear Iga Bullous Dermatosis Antigen97 Kda Lad Antigen97-LadLinear Iga Bullous Disease Antigen Of 97 KdaLabd97
Cell JunctionHemidesmosomeMembraneSingle-Pass Type Ii Membrane ProteinLocalized Along The Plasma Membrane Of The Hemidesmosome120 Kda Linear Iga Disease Antigen: SecretedExtracellular SpaceExtracellular MatrixBasement MembraneExclusively Localized To Anchoring FilamentsLocalized To The Epidermal Side Of Split Skin97 Kda Linear Iga Disease Antigen: SecretedLocalized In The Lamina Lucida Beneath The Hemidesmosomes
Alternative ELISA Names
Collagen Alpha-1(Xvii Chain ELISA kit180 Kda Bullous Pemphigoid Antigen 2 ELISA kitBullous Pemphigoid Antigen 2 Cleaved Into - 120 Kda Linear Iga Disease Antigen ELISA kit120 Kda Linear Iga Dermatosis Antigen ELISA kitLinear Iga Disease Antigen 1 ELISA kitLad-1 - 97 Kda Linear Iga Disease Antigen ELISA kit97 Kda Linear Iga Bullous Dermatosis Antigen ELISA kit97 Kda Lad Antigen ELISA kit97-Lad ELISA kitLinear Iga Bullous Disease Antigen Of 97 Kda ELISA kitLabd97 ELISA kitCOL17A1 ELISA kitBP180 ELISA kitBPAG2 ELISA kit
Specificity
This kit recognizes Human COL17 in samples. No significant cross-reactivity or interference between Human COL17 and analogues was observed.
