| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.057ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This ChT Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | SLC5A7 |
| Gene ID: | 60482 |
| Uniprot ID: | SC5A7_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in putamen, spinal cord and medulla. Expressed in cholinergic neurons. |
| Post Translational Modifications | Phosphorylated. |
| Function | High-affinity Na(+)-coupled choline transmembrane symporter. Functions as an electrogenic, voltage-dependent transporter with variable charge/choline stoichiometry. Choline uptake and choline-induced current is also Cl(-)-dependent where Cl(-) is likely a regulatory ion rather than cotransported ion. Plays a critical role in acetylcholine (ACh) synthesis by taking up the substrate choline from the synaptic cleft into the presynaptic nerve terminals after neurotransmitter release. SLC5A7/CHT1-mediated choline high-affinity transport in cholinergic neurons is the rate-limiting step for production of ACh, thereby facilitating communication by subsequent action potentials. Localized predominantly in presynaptic terminal intracellular organelles, and translocated to the plasma membrane in active form in response to neuronal activity. |
| Protein Name | High Affinity Choline Transporter 1 Hcht1 Hemicholinium-3-Sensitive Choline Transporter Cht Solute Carrier Family 5 Member 7 |
| Database Links | Reactome: R-HSA-264642 Reactome: R-HSA-425366 Reactome: R-HSA-5619114 Reactome: R-HSA-5658471 |
| Cellular Localisation | Presynaptic Cell Membrane Multi-Pass Membrane Protein Cell Projection Axon Early Endosome Membrane Cytoplasmic Vesicle Secretory Vesicle Synaptic Vesicle Membrane Localized At The Neuromuscular Junction Localization At The Plasma Membrane Is Transient Due To The Rapid Endocytosis Of Slc5a7/Cht1 Via The Clathrin-Mediated Pathway Where It Localizes To Early Endosomes Also Localized To Synaptic Vesicles Where It Is Likely Mobilized To The Cell Surface By Exocytosis |
| Alternative ELISA Names | High Affinity Choline Transporter 1 ELISA kit Hcht1 ELISA kit Hemicholinium-3-Sensitive Choline Transporter ELISA kit Cht ELISA kit Solute Carrier Family 5 Member 7 ELISA kit SLC5A7 ELISA kit CHT1 ELISA kit |
| output |
Information sourced from Uniprot.org
