Human CHST4 protein (Recombinant-Active) (N-His) (STJP012206)

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STJP012206-100
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Covered by St John's Laboratory Guarantee

Host: Mammalian Cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Recombinant-Active-Human CHST4-N-His protein was developed from mammalian cells and has a target region of N-His. For use in research applications.
Formulation: Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Dilution Range: Reconstitute in sterile water for a stock solution.
Storage Instruction: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt.
Endotoxin: <0.1 EU/Mu g of the protein by the LAL method.
Gene Symbol: CHST4
Gene ID: 10164
Uniprot ID: CHST4_HUMAN
Immunogen Region: His29-His386
Immunogen: Homo sapiens (Human)
Function Sulfotransferase involved in SELL/L-selectin ligand biosynthesis pathway. Catalyzes the transfer of the sulfate group from 3'-phospho-5'-adenylyl sulfate (PAPS) onto the hydroxyl group at C-6 position of the non-reducing N-acetylglucosamine (GlcNAc) residue within O-linked mucin-type glycans. Contributes to generate sialyl 6-sulfo Lewis X determinant (also known as MECA-79 epitope) for SELL recognition, a prerequisite for continuous lymphocyte homing into peripheral lymph nodes and antigen immune surveillance. Transfers the sulfate group primarily on core 2 GlcNAcbeta1-6(Galbeta1-3)GalNAcalphaSer/Thr and extended core 1 GlcNAcbeta1-3Galbeta1-3GalNAcalphaSer/Thr based O-linked glycans on CD34 and GLYCAM1 peripheral node addressins (PNAds) expressed on the lumenal side of high endothelial venules (HEVs). The recognition of PNAds by SELL initiates a multistep process comprising tethering and rolling of blood lymphocytes on HEVs against the blood flow, followed by chemokine signaling, integrin-mediated lymphocyte adhesion onto endothelial cells and lymphocyte transendothelial migration. Modulates rolling velocity and differential T and B lymphocyte recruitment into peripheral lymph nodes, with a major role in B lymphocyte homing. Might be redundant in sulfation of MADCAM1 and lymphocyte trafficking to mesenteric lymph nodes. Can also sulfonate core 3 GlcNAcbeta1-3GalNAc-R based glycans as well as GlcNAcbeta1-3Galbeta1-Glc, GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharides, which might be ectopically expressed during tumorigenesis.
Protein Name Carbohydrate Sulfotransferase 4
Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 3
Gst-3
High Endothelial Cells N-Acetylglucosamine 6-O-Sulfotransferase
Hec-Glcnac6st
L-Selectin Ligand Sulfotransferase
Lsst
N-Acetylglucosamine 6-O-Sulfotransferase 2
Glcnac6st-2
Gn6st-2
Database Links Reactome: R-HSA-913709
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Protein Names Carbohydrate Sulfotransferase 4 protein
Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 3 protein
Gst-3 protein
High Endothelial Cells N-Acetylglucosamine 6-O-Sulfotransferase protein
Hec-Glcnac6st protein
L-Selectin Ligand Sulfotransferase protein
Lsst protein
N-Acetylglucosamine 6-O-Sulfotransferase 2 protein
Glcnac6st-2 protein
Gn6st-2 protein
CHST4 protein

Information sourced from Uniprot.org

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