| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.38ng/mL |
| Detection Limit: | 0.63~40ng/mL |
| Short Description : | This CDKN2A Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | CDKN2A |
| Gene ID: | 1029 |
| Uniprot ID: | CDN2A_HUMAN |
| Specificity: | This kit recognizes Human CDKN2A in samples. No significant cross-reactivity or interference between Human CDKN2A and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylation seems to increase interaction with CDK4. |
| Function | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. |
| Protein Name | Cyclin-Dependent Kinase Inhibitor 2aCyclin-Dependent Kinase 4 Inhibitor ACdk4iMultiple Tumor Suppressor 1Mts-1P16-Ink4aP16-Ink4P16ink4a |
| Database Links | Reactome: R-HSA-111471Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-3108214Reactome: R-HSA-3232118Reactome: R-HSA-6804757Reactome: R-HSA-69541Reactome: R-HSA-8941858Reactome: R-HSA-9645722Reactome: R-HSA-9646303Reactome: R-HSA-9646304Reactome: R-HSA-9759194Reactome: R-HSA-2559582Reactome: R-HSA-69231Reactome: R-HSA-8853884Reactome: R-HSA-9630791Reactome: R-HSA-9630794Reactome: R-HSA-9632697Reactome: R-HSA-9632700Reactome: R-HSA-9825892 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative ELISA Names | Cyclin-Dependent Kinase Inhibitor 2a ELISA kitCyclin-Dependent Kinase 4 Inhibitor A ELISA kitCdk4i ELISA kitMultiple Tumor Suppressor 1 ELISA kitMts-1 ELISA kitP16-Ink4a ELISA kitP16-Ink4 ELISA kitP16ink4a ELISA kitCDKN2A ELISA kitCDKN2 ELISA kitMTS1 ELISA kit |
| Specificity | This kit recognizes Human CDKN2A in samples. No significant cross-reactivity or interference between Human CDKN2A and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-106 | 99 |
| EDTA plasma (n=8) | 95-107 | 101 |
| Cell culture media (n=8) | 86-99 | 91 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.08 | 6.28 | 16.73 | 1.92 | 6.51 | 15.76 |
| Standard deviation | 0.11 | 0.37 | 0.80 | 0.11 | 0.37 | 0.68 |
| CV (%) | 5.29 | 5.89 | 4.78 | 5.73 | 5.68 | 4.31 |
