| Host: | E.coli |
| Applications: | ELISA/Immunogen/SDS-PAGE/WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human CDK7-N-His protein was developed from e.coli and has a target region of N-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Dilution Range: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin: | Please contact us for further information. |
| Gene Symbol: | CDK7 |
| Gene ID: | 1022 |
| Uniprot ID: | CDK7_HUMAN |
| Immunogen Region: | Met1-Phe346 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Phosphorylation of Ser-164 during mitosis inactivates the enzyme. Phosphorylation of Thr-170 is required for activity. Phosphorylated at Ser-164 and Thr-170 by CDK2. |
| Function | Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. Initiates transcription by RNA polymerase II by mediating phosphorylation of POLR2A at 'Ser-5' of the repetitive C-terminal domain (CTD) when POLR2A is in complex with DNA, promoting dissociation from DNA and initiation. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the CTD of POLR2A, allowing its escape from the promoter and elongation of the transcripts. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53.this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition. |
| Protein Name | Cyclin-Dependent Kinase 739 Kda Protein KinaseP39 Mo15Cdk-Activating Kinase 1Cell Division Protein Kinase 7Serine/Threonine-Protein Kinase 1Tfiih Basal Transcription Factor Complex Kinase Subunit |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-6807505Reactome: R-HSA-69202Reactome: R-HSA-69231Reactome: R-HSA-69273Reactome: R-HSA-69656Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075Reactome: R-HSA-8939236 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionColocalizes With Prkci In The Cytoplasm And NucleusTranslocates From The Nucleus To Cytoplasm And Perinuclear Region In Response To Dna-Bound Peptides |
| Alternative Protein Names | Cyclin-Dependent Kinase 7 protein39 Kda Protein Kinase proteinP39 Mo15 proteinCdk-Activating Kinase 1 proteinCell Division Protein Kinase 7 proteinSerine/Threonine-Protein Kinase 1 proteinTfiih Basal Transcription Factor Complex Kinase Subunit proteinCDK7 proteinCAK proteinCAK1 proteinCDKN7 proteinMO15 proteinSTK1 protein |
Information sourced from Uniprot.org