Human CDH5/VE-cadherin protein (Recombinant) (C-hFc&His) (STJP001090)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human CDH5/VE-cadherin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp48-Gln593) of human VE-Cadherin (Accession #NP_001786.2) fused with an Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human CDH5/VE-cadherin-C-hFc&His protein was developed in hek293 cells using the region Asp48-Gln593. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CDH5 |
| Gene ID | 1003 |
| Uniprot ID | CADH5_HUMAN |
| Immunogen | Recombinant Human CDH5/VE-cadherin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp48-Gln593) of human VE-Cadherin (Accession #NP_001786.2) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Asp48-Gln593 |
| Immunogen Sequence | DWIWNQMHIDEEKNTSLPHH VGKIKSSVSRKNAKYLLKGE YVGKVFRVDAETGDVFAIER LDRENISEYHLTAVIVDKDT GENLETPSSFTIKVHDVNDN WPVFTHRLFNASVPESSAVG TSVISVTAVDADDPTVGDHA SVMYQILKGKEYFAIDNSGR IITITKSLDREKQARYEIVV EARDAQGLRGDSGTATVLVT LQDINDNFPFFTQTKYTFVV PEDTRVGTSVGSLFVEDPD |
Additional Info
| Tissue Specificity | Expressed in endothelial cells (at protein level). Expressed in the brain. |
| Post Translational Modifications | Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. O-glycosylated. |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1. Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Associates with CTNND1/p120-catenin to control CADH5 endocytosis. |
| Protein Name | Cadherin-57b4 AntigenVascular Endothelial CadherinVe-CadherinCd Antigen Cd144 |
| Database Links | Reactome: R-HSA-418990Reactome: R-HSA-5218920Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell JunctionAdherens JunctionCell MembraneSingle-Pass Type I Membrane ProteinCytoplasmFound At Cell-Cell Boundaries And Probably At Cell-Matrix BoundariesKrit1 And Cdh5 Reciprocally Regulate Their Localization To Endothelial Cell-Cell Junctions |
| Alternative Protein Names | Cadherin-5 protein7b4 Antigen proteinVascular Endothelial Cadherin proteinVe-Cadherin proteinCd Antigen Cd144 proteinCDH5 protein |
Information sourced from Uniprot.org