| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human CD69-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Dilution Range: |
Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Gene Symbol: |
CD69 |
| Gene ID: |
969 |
| Uniprot ID: |
CD69_HUMAN |
| Immunogen Region: |
SER62-LYS199 |
| Immunogen: |
Optimized DNA sequence encoding extracellular domain of Human CD69 (SER62-LYS199) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. |
| Post Translational Modifications | Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. |
| Function | Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types. Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells. Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage. Acts also via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells. Acts as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T-lymphocytes and negatively regulates the inflammatory response by inducing the expression of PDCD1 through the activation of NFAT. Participates in adipose tissue-derived mesenchymal stem cells (ASCs)-mediated protection against P. aeruginosa infection. Mechanistically, specifically recognizes P. aeruginosa to promote ERK1 activation, followed by granulocyte-macrophage colony-stimulating factor (GM-CSF) and other inflammatory cytokines secretion. In eosinophils, induces IL-10 production through the ERK1/2 pathway. Negatively regulates the chemotactic responses of effector lymphocytes and dendritic cells (DCs) to sphingosine 1 phosphate/S1P by acting as a S1PR1 receptor agonist and facilitating the internalization and degradation of the receptor. |
| Protein Name | Early Activation Antigen Cd69Activation Inducer MoleculeAimBl-Ac/P26C-Type Lectin Domain Family 2 Member CEa1Early T-Cell Activation Antigen P60Gp32/28Leukocyte Surface Antigen Leu-23Mlr-3Cd Antigen Cd69 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Protein Names | Early Activation Antigen Cd69 proteinActivation Inducer Molecule proteinAim proteinBl-Ac/P26 proteinC-Type Lectin Domain Family 2 Member C proteinEa1 proteinEarly T-Cell Activation Antigen P60 proteinGp32/28 proteinLeukocyte Surface Antigen Leu-23 proteinMlr-3 proteinCd Antigen Cd69 proteinCD69 proteinCLEC2C protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
