Human CD69 protein (Recombinant) (C-His) (STJP000853)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human CD69 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser 62-Lys 199 ) of human CD69 (Accession #NP_001772.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human CD69-C-His protein was developed in hek293 cells using the region Ser 62-Lys 199. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CD69 |
| Gene ID | 969 |
| Uniprot ID | CD69_HUMAN |
| Immunogen | Recombinant Human CD69 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser 62-Lys 199 ) of human CD69 (Accession #NP_001772.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Ser 62-Lys 199 |
| Immunogen Sequence | SVGQYNCPGQYTFSMPSDSH VSSCSEDWVGYQRKCYFIST VKRSWTSAQNACSEHGATLA VIDSEKDMNFLKRYAGREEH WVGLKKEPGHPWKWSNGKEF NNWFNVTGSDKCVFLKNTEV SSMECEKNLYWICNKPYK |
Additional Info
| Tissue Specificity | Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. |
| Post Translational Modifications | Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. |
| Function | Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types. Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells. Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage. Also acts via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells. Acts as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T-lymphocytes and negatively regulates the inflammatory response by inducing the expression of PDCD1 through the activation of NFAT. Participates in adipose tissue-derived mesenchymal stem cells (ASCs)-mediated protection against P.aeruginosa infection. Mechanistically, specifically recognizes P.aeruginosa to promote ERK1 activation, followed by granulocyte-macrophage colony-stimulating factor (GM-CSF) and other inflammatory cytokines secretion. In eosinophils, induces IL-10 production through the ERK1/2 pathway. Negatively regulates the chemotactic responses of effector lymphocytes and dendritic cells (DCs) to sphingosine 1 phosphate/S1P by acting as a S1PR1 receptor agonist and facilitating the internalization and degradation of the receptor. |
| Protein Name | Early Activation Antigen Cd69Activation Inducer MoleculeAimBl-Ac/P26C-Type Lectin Domain Family 2 Member CEa1Early T-Cell Activation Antigen P60Gp32/28Leukocyte Surface Antigen Leu-23Mlr-3Cd Antigen Cd69 |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Protein Names | Early Activation Antigen Cd69 proteinActivation Inducer Molecule proteinAim proteinBl-Ac/P26 proteinC-Type Lectin Domain Family 2 Member C proteinEa1 proteinEarly T-Cell Activation Antigen P60 proteinGp32/28 proteinLeukocyte Surface Antigen Leu-23 proteinMlr-3 proteinCd Antigen Cd69 proteinCD69 proteinCLEC2C protein |
Information sourced from Uniprot.org