| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.56ng/mL |
| Detection Limit: | 0.94~60ng/mL |
| Short Description: | This CD38 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | CD38 |
| Gene ID: | 952 |
| Uniprot ID: | CD38_HUMAN |
| Specificity: | This kit recognizes Human cADPRH/CD38 in samples. No significant cross-reactivity or interference between Human cADPRH/CD38 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion. Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+). Has cADPR hydrolase activity. |
| Protein Name | Adp-Ribosyl Cyclase/Cyclic Adp-Ribose Hydrolase 1 2'-Phospho-Adp-Ribosyl Cyclase 2'-Phospho-Adp-Ribosyl Cyclase/2'-Phospho-Cyclic-Adp-Ribose Transferase 2'-Phospho-Cyclic-Adp-Ribose Transferase Adp-Ribosyl Cyclase 1 Adprc 1 Cyclic Adp-Ribose Hydrolase 1 Cadpr Hydrolase 1 T10 Cd Antigen Cd38 |
| Database Links | Reactome: R-HSA-196807 |
| Cellular Localisation | Cell Surface Membrane Single-Pass Type Ii Membrane Protein |
| Alternative ELISA Names | Adp-Ribosyl Cyclase/Cyclic Adp-Ribose Hydrolase 1 ELISA kit 2'-Phospho-Adp-Ribosyl Cyclase ELISA kit 2'-Phospho-Adp-Ribosyl Cyclase/2'-Phospho-Cyclic-Adp-Ribose Transferase ELISA kit 2'-Phospho-Cyclic-Adp-Ribose Transferase ELISA kit Adp-Ribosyl Cyclase 1 ELISA kit Adprc 1 ELISA kit Cyclic Adp-Ribose Hydrolase 1 ELISA kit Cadpr Hydrolase 1 ELISA kit T10 ELISA kit Cd Antigen Cd38 ELISA kit CD38 ELISA kit |
| Specificity | This kit recognizes Human cADPRH/CD38 in samples. No significant cross-reactivity or interference between Human cADPRH/CD38 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-99 | 93 |
| EDTA plasma (n=8) | 95-111 | 101 |
| Cell culture media (n=8) | 95-110 | 101 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 3.15 | 6.75 | 27.89 | 3.33 | 6.48 | 29.83 |
| Standard deviation | 0.16 | 0.39 | 1.52 | 0.20 | 0.33 | 1.34 |
| CV (%) | 5.08 | 5.78 | 5.45 | 6.01 | 5.09 | 4.49 |
