Human CD31/PECAM1 protein (Recombinant-Active) (C-His) (STJP011888)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human CD31/PECAM1-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by the ability of the Immobilised protein to support the adhesion of human acute T cell leukemia cells (Jurkat). When 8×10^4 cells/well are added to CD31/PECAM1 coated plates (5 Mu g/mL, 100 Mu L/well) in the presence of 10 Mu g/mL PHA, appr |
Target Information
| Gene Symbol | PECAM1 |
| Gene ID | 5175 |
| Uniprot ID | PECA1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gln28-Lys601 |
Additional Info
| Post Translational Modifications | Phosphorylated on Ser and Tyr residues after cellular activation by src kinases. Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690. Constitutively phosphorylated on Ser-734 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. |
| Function | Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells.PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis. |
| Protein Name | Platelet Endothelial Cell Adhesion MoleculePecam-1EndocamGpiia'Peca1Cd Antigen Cd31 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-202733Reactome: R-HSA-210990Reactome: R-HSA-216083Reactome: R-HSA-432142Reactome: R-HSA-6798695Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell Surface Expression On Neutrophils Is Down-Regulated Upon Fmlp Or Cxcl8/Il8-Mediated StimulationIsoform Long: Cell MembraneMembrane RaftCell JunctionLocalizes To The Lateral Border Recycling Compartment (Lbrc) And Recycles From The Lbrc To The Junction In Resting Endothelial CellsIsoform Delta15: Cell Junction |
| Alternative Protein Names | Platelet Endothelial Cell Adhesion Molecule proteinPecam-1 proteinEndocam proteinGpiia' proteinPeca1 proteinCd Antigen Cd31 proteinPECAM1 protein |
Information sourced from Uniprot.org