| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 1.0 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Anti-CD3 epsilon/CD3e Antibody at 1:3000 (100 Mu L/well) can bind recombinant Human CD3E, the EC50 of Human CD3E is 0.48 ng/mL. |
| Gene Symbol: |
CD3E |
| Gene ID: |
916 |
| Uniprot ID: |
CD3E_HUMAN |
| Immunogen Region: |
Met1-Asp126 |
| Immunogen: |
Recombinant Human CD3 epsilon/CD3E Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Asp126) of human CD3 epsilon/CD3e (Accession #NP_000724.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: |
MQSGTHWRVLGLCLLSVGVW GQDGNEEMGGITQTPYKVSI SGTTVILTCPQYPGSEILWQ HNDKNIGGDEDDKNIGSDED HLSLKEFSELEQSGYYVCYP RGSKPEDANFYLYLRARVCE NCMEMD |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx. |
| Protein Name | T-Cell Surface Glycoprotein Cd3 Epsilon ChainT-Cell Surface Antigen T3/Leu-4 Epsilon ChainCd Antigen Cd3e |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-202424Reactome: R-HSA-202427Reactome: R-HSA-202430Reactome: R-HSA-202433Reactome: R-HSA-389948 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | T-Cell Surface Glycoprotein Cd3 Epsilon Chain proteinT-Cell Surface Antigen T3/Leu-4 Epsilon Chain proteinCd Antigen Cd3e proteinCD3E proteinT3E protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
