Host: | HEK293 |
Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Recombinant-Human CD3 Delta-protein was developed from hek293. For use in research applications. |
Conjugation: | Unconjugated |
Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
Gene Symbol: | CD3D |
Gene ID: | 915 |
Uniprot ID: | CD3D_HUMAN |
Immunogen Region: | ECD |
Immunogen: | Optimized DNA sequence encoding extracellular domain of Human CD3 Delta including a C-terminal His tag, were expressed in HEK293 cells. NA |
Tissue Specificity | CD3D is mostly present on T-lymphocytes with its TCR-CD3 partners. Present also in fetal NK-cells. |
Post Translational Modifications | Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. |
Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells. |
Protein Name | T-Cell Surface Glycoprotein Cd3 Delta Chain T-Cell Receptor T3 Delta Chain Cd Antigen Cd3d |
Database Links | Reactome: R-HSA-198933 Reactome: R-HSA-202424 Reactome: R-HSA-202427 Reactome: R-HSA-202430 Reactome: R-HSA-202433 Reactome: R-HSA-389948 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
Alternative Protein Names | T-Cell Surface Glycoprotein Cd3 Delta Chain protein T-Cell Receptor T3 Delta Chain protein Cd Antigen Cd3d protein CD3D protein T3D protein |
Information sourced from Uniprot.org