Human CD177 protein (Recombinant) (C-hFc&His) (STJP001213)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human CD177 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gly407) of human CD177 (Accession #NP_065139.2.) fused with a Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human CD177-C-hFc&His protein was developed in hek293 cells using the region Met1-Gly407. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human PDPN at 5 Mu g/mL (100 Mu L/well) can bind Human CD177 with a linear range of 6.8-250.6 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CD177 |
| Gene ID | 57126 |
| Uniprot ID | CD177_HUMAN |
| Immunogen | Recombinant Human CD177 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gly407) of human CD177 (Accession #NP_065139.2.) fused with a Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Met1-Gly407 |
| Immunogen Sequence | MSPVLLLALLGFILPLPGVQ ALLCQFGTVQHVWKVSDLPR QWTPKNTSCDSGLGCQDTLM LIESGPQVSLVLSKGCTEAK DQEPRVTEHRMGPGLSLISY TFVCRQEDFCNNLVNSLPLW APQPPADPGSLRCPVCLSME GCLEGTTEEICPKGTTHCYD GLLRLRGGGIFSNLRVQGCM PQPGCNLLNGTQEIGPVGMT ENCNRKDFLTCHRGTTIMTH GNLAQEPTDWTTSNTEMCE |
Additional Info
| Tissue Specificity | Highly expressed in normal bone marrow and weakly expressed in fetal liver. During neutrophil differentiation, expression begins at the metamyelocyte stage and continues throughout the subsequent stages (at protein level). Expressed by a subset of mature neutrophils (at protein level). The percentage of neutrophils expressing CD177 varies across the population. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). |
| Post Translational Modifications | N-glycosylated. A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). |
| Function | In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production. In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration. Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro. However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo. Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils. By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis. |
| Protein Name | Cd177 AntigenHuman Neutrophil Alloantigen 2aHna-2aNb1 GlycoproteinNb1 GpPolycythemia Rubra Vera Protein 1Prv-1Cd Antigen Cd177 |
| Database Links | Reactome: R-HSA-140875Reactome: R-HSA-202733Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorMembrane RaftGpi-Like-AnchorSecretedCytoplasmic Granule MembraneCell ProjectionLamellipodiumCell Surface Expression On Neutrophils Is Increased Upon Tnf-AlphaFmlp Or Cxcl8/Il8-Mediated StimulationIn NeutrophilsStored Predominantly In Secondary And Tertiary GranulesCan Also Be Shedded From The Cell MembraneLocalizes To Lamellar Protrusions In Spreading Neutrophils |
| Alternative Protein Names | Cd177 Antigen proteinHuman Neutrophil Alloantigen 2a proteinHna-2a proteinNb1 GlycoproteinNb1 Gp proteinPolycythemia Rubra Vera Protein 1 proteinPrv-1 proteinCd Antigen Cd177 proteinCD177 proteinNB1 proteinPRV1 proteinUNQ595 proteinPRO1181 protein |
Information sourced from Uniprot.org