| Host: | HEK293 |
| Applications: | ELISA/WB |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Recombinant-Human CD16b (FcgR3b)-protein was developed from hek293. For use in research applications. |
| Conjugation: | Biotin |
| Formulation: | Lyophilised 0.2 Mu m filtered PBS solution, pH7.4 containing 5% trehalose. |
| Dilution Range: | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for twelve months. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Gene Symbol: | FCGR3B |
| Gene ID: | 2215 |
| Uniprot ID: | FCG3B_HUMAN |
| Immunogen Region: | ECD |
| Immunogen: | Optimized DNA sequence encoding Human extracellular domain of human CD16bincluding a C-terminal 6His tag was expressed in HEK293 cells. CD16b was further labled by Biotin for detection by avidin conjugated secondary probes NA |
| Tissue Specificity | Expressed specifically by polymorphonuclear leukocytes (neutrophils). Also expressed by stimulated eosinophils. |
| Post Translational Modifications | Glycosylated. Glycosylation plays an inhibitory role in the interaction with IgG3. The soluble form is produced by a proteolytic cleavage. |
| Function | Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils. |
| Protein Name | Low Affinity Immunoglobulin Gamma Fc Region Receptor Iii-B Fc-Gamma Riii-Beta Cd16-I Fc-Gamma Riii Fc-Gamma Riiib Fcriii Fcriiib Fcr-10 Igg Fc Receptor Iii-1 Cd Antigen Cd16b |
| Database Links | Reactome: R-HSA-163125 Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Gpi-Anchor Secreted Secreted After Cleavage |
| Alternative Protein Names | Low Affinity Immunoglobulin Gamma Fc Region Receptor Iii-B protein Fc-Gamma Riii-Beta protein Cd16-I protein Fc-Gamma Riii protein Fc-Gamma Riiib protein Fcriii protein Fcriiib protein Fcr-10 protein Igg Fc Receptor Iii-1 protein Cd Antigen Cd16b protein FCGR3B protein CD16B protein FCG3 protein FCGR3 protein IGFR3 protein |
Information sourced from Uniprot.org
