| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human CD150 (SLAM)-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
0.2 Mu m filtered PBS solution, pH7.2. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The actiity of recombinant SLAM (CD150) was measured by its ability to bind biotinylated recombinant human SH2D1A in a functional ELISA. NA |
| Gene Symbol: |
SLAMF1 |
| Gene ID: |
6504 |
| Uniprot ID: |
SLAF1_HUMAN |
| Immunogen Region: |
ECD |
| Immunogen: |
Optimized DNA sequence encoding Human extracellular domain of human SLAM (CD150) including a C-terminal 6His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. Activated B-cells express isoform 1, isoform 3 and a cytoplasmic isoform. Isoform 4 is expressed in B-cells, primary T-cells, dendritic cells and macrophages. Isoform 4 is expressed in tumors of the central nervous system. |
| Post Translational Modifications | Phosphorylated on tyrosine residues by FYN. |
| Function | Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. SLAMF1-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAMF1 signaling seem to exist: one depending on SH2D1A (and perhaps SH2D1B) and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. Initially it has been proposed that association with SH2D1A prevents binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2. However, signaling is also regulated by SH2D1A which can simultaneously interact with and recruit FYN which subsequently phosphorylates and activates SLAMF1. Mediates IL-2-independent proliferation of activated T-cells during immune responses and induces IFN-gamma production. Downstreaming signaling involves INPP5D, DOK1 and DOK2 leading to inhibited IFN-gamma production in T-cells, and PRKCQ, BCL10 and NFKB1 leading to increased T-cell activation and Th2 cytokine production. Promotes T-cell receptor-induced IL-4 secretion by CD4(+) cells. Inhibits antigen receptor-mediated production of IFN-gamma, but not IL-2, in CD4(-)/CD8(-) T-cells. Required for IL-4 production by germinal centers T follicular helper (T(Fh))cells. May inhibit CD40-induced signal transduction in monocyte-derived dendritic cells. May play a role in allergic responses and may regulate allergen-induced Th2 cytokine and Th1 cytokine secretion. In conjunction with SLAMF6 controls the transition between positive selection and the subsequent expansion and differentiation of the thymocytic natural killer T (NKT) cell lineage. Involved in the peripheral differentiation of indifferent natural killer T (iNKT) cells toward a regulatory NKT2 type. In macrophages involved in down-regulation of IL-12, TNF-alpha and nitric oxide in response to lipopolysaccharide (LPS). In B-cells activates the ERK signaling pathway independently of SH2D1A but implicating both, SYK and INPP5D, and activates Akt signaling dependent on SYK and SH2D1A. In B-cells also activates p38 MAPK and JNK1 and JNK2. In conjunction with CD84/SLAMF5 and SLAMF6 may be a negative regulator of the humoral immune response. Involved in innate immune response against Gram-negative bacteria in macrophages.probably recognizes OmpC and/or OmpF on the bacterial surface, regulates phagosome maturation and recruitment of the PI3K complex II (PI3KC3-C2) leading to accumulation of PdtIns(3)P and NOX2 activity in the phagosomes. (Microbial infection) Acts as a receptor for Measles virus.also including isoform 4. |
| Protein Name | Signaling Lymphocytic Activation MoleculeCdw150Ipo-3Slam Family Member 1Cd Antigen Cd150 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinPresent On The Surface Of B-Cells And T-CellsLocated At The Plasma Membrane Contacts Between Neighboring T-CellsIsoform 3: SecretedIsoform 4: Cell MembraneOverexpressed Isoform 4 Is Detected On The Cell SurfaceIn Glioma Cell Lines Endogenuous Isoform 4 Is Detetced Predominantly In The Cytoplasm And Colocalized With Endoplasmic Reticulum And Golgi Markers |
| Alternative Protein Names | Signaling Lymphocytic Activation Molecule proteinCdw150 proteinIpo-3 proteinSlam Family Member 1 proteinCd Antigen Cd150 proteinSLAMF1 proteinSLAM protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
