Human CCN2 (CCN family member 2) Sandwich ELISA Kit (STJE0002508)

SPECIFICATIONS
STJE0002508
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General Information

Short DescriptionThis CCN2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityHuman
Sensitivity37.5pg/mL
Detection Limit62.5~4000pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolCCN2
Gene ID1490
Uniprot IDCCN2_HUMAN
SpecificityThis kit recognizes Human CTGF in samples. No significant cross-reactivity or interference between Human CTGF and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Function Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Protein Name Ccn Family Member 2
Cellular Communication Network Factor 2
Connective Tissue Growth Factor
Hypertrophic Chondrocyte-Specific Protein 24
Insulin-Like Growth Factor-Binding Protein 8
Ibp-8
Igf-Binding Protein 8
Igfbp-8
Database Links Reactome: R-HSA-2032785
Reactome: R-HSA-8951671
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative ELISA Names Ccn Family Member 2 ELISA kit
Cellular Communication Network Factor 2 ELISA kit
Connective Tissue Growth Factor ELISA kit
Hypertrophic Chondrocyte-Specific Protein 24 ELISA kit
Insulin-Like Growth Factor-Binding Protein 8 ELISA kit
Ibp-8 ELISA kit
Igf-Binding Protein 8 ELISA kit
Igfbp-8 ELISA kit
CCN2 ELISA kit
CTGF ELISA kit
HCS24 ELISA kit
IGFBP8 ELISA kit
Specificity This kit recognizes Human CTGF in samples. No significant cross-reactivity or interference between Human CTGF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-104 96
EDTA plasma (n=8) 97-108 102
Cell culture media (n=8) 88-99 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 216.66 518.52 1996.04 223.99 564.64 2118.31
Standard deviation 12.09 27.69 79.64 11.83 23.60 71.18
CV (%) 5.58 5.34 3.99 5.28 4.18 3.36

Citations

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