| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <1 EU/Mu g |
| Immunoreactivity: | 1.Measured by the ability to inhibit the proliferation of HUVEC (Human Umbilical Vein Endothelial Cells).The ED50 for this effect is 0.53-2.12 ng/mL.|2.Measured by its ability to chemoattract THP-1 cells. The ED50 for this effec |
| Gene Symbol: | CCL2 |
| Gene ID: | 6347 |
| Uniprot ID: | CCL2_HUMAN |
| Immunogen: | Active Recombinant Human CCL2/MCP-1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Gln24-Thr99) of human CCL2/MCP-1 (Accession #NP_002973.1) fused with no additional amino acid. |
| Immunogen Sequence: | QPDAINAPVTCCYNFTNRKI SVQRLASYRRITSSKCPKEA VIFKTIVAKEICADPKQKWV QDSMDHLDKQTQTPKT |
| Post Translational Modifications | Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. N-Glycosylated. |
| Function | Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
| Protein Name | C-C Motif Chemokine 2 Hc11 Monocyte Chemoattractant Protein 1 Monocyte Chemotactic And Activating Factor Mcaf Monocyte Chemotactic Protein 1 Mcp-1 Monocyte Secretory Protein Je Small-Inducible Cytokine A2 |
| Database Links | Reactome: R-HSA-380108 Reactome: R-HSA-380994 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-9818026 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | C-C Motif Chemokine 2 protein Hc11 protein Monocyte Chemoattractant Protein 1 protein Monocyte Chemotactic And Activating Factor protein Mcaf protein Monocyte Chemotactic Protein 1 protein Mcp-1 protein Monocyte Secretory Protein Je protein Small-Inducible Cytokine A2 protein CCL2 protein MCP1 protein SCYA2 protein |
Information sourced from Uniprot.org