Human CBLIF (Cobalamin binding intrinsic factor) CLIA Kit (STJC0000235)

Human CBLIF (Cobalamin binding intrinsic factor) CLIA Kit (STJC0000235)

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STJC0000235
£180.50 - £462.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 0.63~40ng/mL
Short Description : This human CBLIF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CBLIF
Gene ID: 2694
Uniprot ID: IF_HUMAN
Specificity: This kit recognizes Human GIF in samples. No significant cross-reactivity or interference between Human GIF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Gastric mucosa.
Function Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis.
Protein Name Cobalamin Binding Intrinsic Factor
Gastric Intrinsic Factor
Intrinsic Factor
If
Inf
Database Links Reactome: R-HSA-3359457
Reactome: R-HSA-3359462
Reactome: R-HSA-3359463
Reactome: R-HSA-9758881
Cellular Localisation Secreted
Alternative CLIA Names Cobalamin Binding Intrinsic Factor CLIA kit
Gastric Intrinsic Factor CLIA kit
Intrinsic Factor CLIA kit
If CLIA kit
Inf CLIA kit
CBLIF CLIA kit
GIF CLIA kit
IFMH CLIA kit
Specificity This kit recognizes Human GIF in samples. No significant cross-reactivity or interference between Human GIF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 92
EDTA plasma(n=8) 95-112 102
Cell culture media(n=8) 100-114 108

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.83 6.37 16.25 1.93 6.42 16.73
Standard deviation 0.18 0.45 1.58 0.20 0.65 1.60
CV (%) 9.84 7.06 9.72 10.36 10.12 9.56

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