| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >747 pmol/min/Mu g. |
| Gene Symbol: | CTSD |
| Gene ID: | 1509 |
| Uniprot ID: | CATD_HUMAN |
| Immunogen Region: | Leu21-Leu412 |
| Immunogen: | Recombinant Human Cathepsin D Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu21-Leu412) of human Cathepsin D/CTSD (Accession #NP_001900.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | LVRIPLHKFTSIRRTMSEVG GSVEDLIAKGPVSKYSQAVP AVTEGPIPEVLKNYMDAQYY GEIGIGTPPQCFTVVFDTGS SNLWVPSIHCKLLDIACWIH HKYNSDKSSTYVKNGTSFDI HYGSGSLSGYLSQDTVSVPC QSASSASALGGVKVERQVFG EATKQPGITFIAAKFDGILG MAYPRISVNNVLPVFDNLMQ QKLVDQNIFSFYLSRDPDAQ PGGELMLGGTDSKYYKGSL |
| Tissue Specificity | Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level). |
| Post Translational Modifications | N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified. |
| Function | Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. |
| Protein Name | Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-2022377Reactome: R-HSA-2132295Reactome: R-HSA-6798695Reactome: R-HSA-77387Reactome: R-HSA-9018519 |
| Cellular Localisation | LysosomeMelanosomeSecretedExtracellular SpaceIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvIn Aortic SamplesDetected As An Extracellular Protein Loosely Bound To The Matrix |
| Alternative Protein Names | Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain proteinCTSD proteinCPSD protein |
Information sourced from Uniprot.org
