Human Cathepsin B protein (Recombinant) (STJP000382)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding mature chain of human Cathepsin B (ARG18-ILE339) including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human Cathepsin B-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. NA |
| Formulation | Lyophilized from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Recombinant Cathepsin B can be stored in working aliquots at 2Β°C-8Β°C C for one month, or at-20Β°C to-70Β°Cfor twelve months. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/Β΅g (1EU/Β΅g). NA |
| Immunoreactivity | The activity was tested by the ability to cleave a flourogenic substrate, the measured activity is 3 nM/ug/min |
Target Information
| Gene Symbol | CTSB |
| Gene ID | 1508 |
| Uniprot ID | CATB_HUMAN |
| Immunogen | Optimized DNA sequence encoding mature chain of human Cathepsin B (ARG18-ILE339) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | ARG18-ILE339 |
Additional Info
| Tissue Specificity | Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum. |
| Function | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis. |
| Protein Name | Cathepsin BApp SecretaseAppsCathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1679131Reactome: R-HSA-2022090Reactome: R-HSA-2132295Reactome: R-HSA-6798695 |
| Cellular Localisation | LysosomeMelanosomeSecretedExtracellular SpaceApical Cell MembranePeripheral Membrane ProteinExtracellular SideIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocalizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells |
| Alternative Protein Names | Cathepsin B proteinApp Secretase proteinApps proteinCathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain proteinCTSB proteinCPSB protein |
Information sourced from Uniprot.org