| Host: |
E. coli |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Catenin beta-1-C-His protein was developed from e. coli and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of 50mM TRIS, 150mM NaCl, pH 8.0. Contact us for customized product form or formulation. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Human CD31 at 1 Mu g/mL (100 Mu L/well) can bind Human CTNNB1 with a linear range of 0.3-4.9 Mu g/ml. |
| Gene Symbol: |
CTNNB1 |
| Gene ID: |
1499 |
| Uniprot ID: |
CTNB1_HUMAN |
| Immunogen Region: |
Met1-Leu781 |
| Immunogen: |
Active Recombinant Human Catenin beta-1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Leu781) of human Beta-catenin (Accession #XP_016861227.1) fused with a 6×His tag at the C-terminus. |
| Tissue Specificity | Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level). |
| Post Translational Modifications | Phosphorylation at Ser-552 by AMPK promotes stabilization of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation. Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity. Phosphorylation by SRC at Tyr-333 promotes interaction with isoform M2 of PKM (PKM2).promoting transcription activation. Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. Ubiquitinated and degraded following interaction with SOX9. S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions. O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1. Deacetylated at Lys-49 by SIRT1. Phosphorylated at Thr-556 by herpes virus 1/HHV-1 leading to CTNNB1 inhibition. |
| Function | Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle. Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4. Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts. |
| Protein Name | Catenin Beta-1Beta-Catenin |
| Database Links | Reactome: R-HSA-195253Reactome: R-HSA-196299Reactome: R-HSA-201681Reactome: R-HSA-201722Reactome: R-HSA-3134973Reactome: R-HSA-351906Reactome: R-HSA-3769402Reactome: R-HSA-381771Reactome: R-HSA-4086398Reactome: R-HSA-418990Reactome: R-HSA-4411364Reactome: R-HSA-4641262Reactome: R-HSA-5218920Reactome: R-HSA-525793Reactome: R-HSA-5339716Reactome: R-HSA-5358747Reactome: R-HSA-5358749Reactome: R-HSA-5358751Reactome: R-HSA-5358752Reactome: R-HSA-5626467Reactome: R-HSA-8853884Reactome: R-HSA-8876493Reactome: R-HSA-8951430Reactome: R-HSA-9733709Reactome: R-HSA-9754189Reactome: R-HSA-9762292Reactome: R-HSA-9764302Reactome: R-HSA-9793380Reactome: R-HSA-9796292Reactome: R-HSA-9823730Reactome: R-HSA-9824272Reactome: R-HSA-9833576 |
| Cellular Localisation | CytoplasmNucleusCytoskeletonCell JunctionAdherens JunctionCell MembraneMicrotubule Organizing CenterCentrosomeSpindle PoleSynapseCilium Basal BodyColocalized With Rapgef2 And Tjp1 At Cell-Cell ContactsCytoplasmic When It Is Un-Stable (Highly Phosphorylated) Or Bound To Cdh1Translocates To The Nucleus When It Is Stabilized (Low Level Of Phosphorylation)Interaction With Glis2 And Muc1 Promotes Nuclear TranslocationInteraction With Emd Inhibits Nuclear LocalizationThe Majority Of Beta-Catenin Is Localized To The Cell MembraneIn InterphaseColocalizes With Crocc Between Cep250 Puncta At The Proximal End Of CentriolesAnd This Localization Is Dependent On Crocc And Cep250In MitosisWhen Nek2 Activity IncreasesIt Localizes To Centrosomes At Spindle Poles Independent Of CroccColocalizes With Cdk5 In The Cell-Cell Contacts And Plasma Membrane Of Undifferentiated And Differentiated Neuroblastoma CellsInteraction With Fam53b Promotes Translocation To The Nucleus |
| Alternative Protein Names | Catenin Beta-1 proteinBeta-Catenin proteinCTNNB1 proteinCTNNB proteinOK proteinSW-cl.35 proteinPRO2286 protein |
Information sourced from Uniprot.org
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