Human Brain Natriuretic Peptide/BNP protein (Recombinant) (C-hFC) (STJP002653)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Brain Natriuretic Peptide/BNP Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His27-Pro104) of human Brain Natriuretic Peptide/BNP (Accession #P16860) fused with a hFC tag at the
General Information
| Short Description | Recombinant-Human Brain Natriuretic Peptide/BNP-C-hFC protein was developed in hek293 cells using the region His27-Pro104. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.2 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.01 EU/Mu g |
Target Information
| Gene Symbol | NPPB |
| Gene ID | 4879 |
| Uniprot ID | ANFB_HUMAN |
| Immunogen | Recombinant Human Brain Natriuretic Peptide/BNP Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His27-Pro104) of human Brain Natriuretic Peptide/BNP (Accession #P16860) fused with a hFC tag at the |
| Immunogen Region | His27-Pro104 |
| Immunogen Sequence | HPLGSPGSASDLETSGLQEQ RNHLQGKLSELQVEQTSLEP LQESPRPTGVWKSREVATEG IRGHRKMVLYTLRAPRSP |
Additional Info
| Post Translational Modifications | The precursor molecule is proteolytically cleaved by the endoproteases FURIN or CORIN at Arg-102 to produce brain natriuretic peptide 32 and NT-proBNP. This likely occurs after it has been secreted into the blood, either during circulation or in the target cells. CORIN also cleaves the precursor molecule at additional residues including Arg-99 and possibly Lys-105. In patients with heart failure, processing and degradation of natriuretic peptides B occurs but is delayed, possibly due to a decrease in enzyme level or activity of CORIN and DPP4. Brain natriuretic peptide 32: Undergoes further proteolytic cleavage by various proteases such as DPP4, MME and possibly FAP, to give rise to a variety of shorter peptides. Cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro). Degraded by IDE. During IDE degradation, the resulting products initially increase the activation of NPR1 and can also stimulate NPR2 to produce cGMP before the fragments are completely degraded and inactivated by IDE (in vitro). O-glycosylated on at least seven residues. In cardiomyocytes, glycosylation at Thr-97 is essential for the stability and processing of the extracellular natriuretic peptides B. Glycosylation, especially at Thr-97, may also be important for brain natriuretic peptide 32 stability and/or extracellular distribution. Glycosylation at Thr-97 appears to inhibit FURIN- or CORIN-mediated proteolytic processing, at least in HEK293 cells. |
| Function | Brain natriuretic peptide 32: Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3. NT-proBNP: May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32. BNP(3-32): May have a role in cardio-renal homeostasis. Able to promote the production of cGMP. |
| Protein Name | Natriuretic Peptides BBrain Natriuretic Factor ProhormonePreprobnpProbnpGamma-Brain Natriuretic PeptideIso-Anp Cleaved Into - Nt-ProbnpNt-Pro-BnpNt-Probnp(1-76 - Probnp(3-108 - Brain Natriuretic Peptide 32Bnp(1-32Bnp-32Brain Natriuretic PeptideBnp - Bnp(1-30 - Bnp(1-29 - Bnp(1-28 - Bnp(2-31 - Bnp(3-32Des-Serpro-BnpProbnp(79-108 - Bnp(3-30 - Bnp(3-29 - Brain Natriuretic Peptide 29Bnp(4-32 - Bnp(4-31 - Bnp(4-30 - Bnp(4-29 - Bnp(4-27 - Bnp(5-32 - Bnp(5-31 - Bnp(5-29 |
| Database Links | |
| Cellular Localisation | Nt-Probnp: SecretedDetected In BloodProbnp(3-108): SecretedBrain Natriuretic Peptide 32: SecretedBnp(3-32): Secreted |
| Alternative Protein Names | Natriuretic Peptides B proteinBrain Natriuretic Factor Prohormone proteinPreprobnp proteinProbnp proteinGamma-Brain Natriuretic Peptide proteinIso-Anp Cleaved Into - Nt-Probnp proteinNt-Pro-Bnp proteinNt-Probnp(1-76 - Probnp(3-108 - Brain Natriuretic Peptide 32 proteinBnp(1-32 proteinBnp-32 proteinBrain Natriuretic Peptide proteinBnp - Bnp(1-30 - Bnp(1-29 - Bnp(1-28 - Bnp(2-31 - Bnp(3-32 proteinDes-Serpro-Bnp proteinProbnp(79-108 - Bnp(3-30 - Bnp(3-29 - Brain Natriuretic Peptide 29 proteinBnp(4-32 - Bnp(4-31 - Bnp(4-30 - Bnp(4-29 - Bnp(4-27 - Bnp(5-32 - Bnp(5-31 - Bnp(5-29 proteinNPPB protein |
Information sourced from Uniprot.org