| Host: |
Mouse/Mouse |
| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
This BNP antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Clonality: |
Monoclonal/Monoclonal |
| Conjugation: |
Unconjugated/Biotin |
| Formulation: |
Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Purification: |
Capture Purification: Protein A.Detection Purification: Protein A. |
| Concentration: |
0.5 mg/mL |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Gene Symbol: |
NPPB |
| Gene ID: |
4879 |
| Uniprot ID: |
ANFB_HUMAN |
| Immunogen: |
Capture immunogen: Recombinant Human BNP protein.Detection Immunogen: Recombinant Human BNP protein. |
| Post Translational Modifications | The precursor molecule is proteolytically cleaved by the endoproteases FURIN or CORIN at Arg-102 to produce brain natriuretic peptide 32 and NT-proBNP. This likely occurs after it has been secreted into the blood, either during circulation or in the target cells. CORIN also cleaves the precursor molecule at additional residues including Arg-99 and possibly Lys-105. In patients with heart failure, processing and degradation of natriuretic peptides B occurs but is delayed, possibly due to a decrease in enzyme level or activity of CORIN and DPP4. Brain natriuretic peptide 32: Undergoes further proteolytic cleavage by various proteases such as DPP4, MME and possibly FAP, to give rise to a variety of shorter peptides. Cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro). Degraded by IDE. During IDE degradation, the resulting products initially increase the activation of NPR1 and can also stimulate NPR2 to produce cGMP before the fragments are completely degraded and inactivated by IDE (in vitro). O-glycosylated on at least seven residues. In cardiomyocytes, glycosylation at Thr-97 is essential for the stability and processing of the extracellular natriuretic peptides B. Glycosylation, especially at Thr-97, may also be important for brain natriuretic peptide 32 stability and/or extracellular distribution. Glycosylation at Thr-97 appears to inhibit FURIN- or CORIN-mediated proteolytic processing, at least in HEK293 cells. |
| Function | Brain natriuretic peptide 32: Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3. NT-proBNP: May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32. BNP(3-32): May have a role in cardio-renal homeostasis. Able to promote the production of cGMP. |
| Protein Name | Natriuretic Peptides BBrain Natriuretic Factor ProhormonePreprobnpProbnpGamma-Brain Natriuretic PeptideIso-Anp Cleaved Into - Nt-ProbnpNt-Pro-BnpNt-Probnp(1-76 - Probnp(3-108 - Brain Natriuretic Peptide 32Bnp(1-32Bnp-32Brain Natriuretic PeptideBnp - Bnp(1-30 - Bnp(1-29 - Bnp(1-28 - Bnp(2-31 - Bnp(3-32Des-Serpro-BnpProbnp(79-108 - Bnp(3-30 - Bnp(3-29 - Brain Natriuretic Peptide 29Bnp(4-32 - Bnp(4-31 - Bnp(4-30 - Bnp(4-29 - Bnp(4-27 - Bnp(5-32 - Bnp(5-31 - Bnp(5-29 |
| Cellular Localisation | Nt-Probnp: SecretedDetected In BloodProbnp(3-108): SecretedBrain Natriuretic Peptide 32: SecretedBnp(3-32): Secreted |
| Alternative Antibody Pair Names | Natriuretic Peptides B antibody pairBrain Natriuretic Factor Prohormone antibody pairPreprobnp antibody pairProbnp antibody pairGamma-Brain Natriuretic Peptide antibody pairIso-Anp Cleaved Into - Nt-Probnp antibody pairNt-Pro-Bnp antibody pairNt-Probnp(1-76 - Probnp(3-108 - Brain Natriuretic Peptide 32 antibody pairBnp(1-32 antibody pairBnp-32 antibody pairBrain Natriuretic Peptide antibody pairBnp - Bnp(1-30 - Bnp(1-29 - Bnp(1-28 - Bnp(2-31 - Bnp(3-32 antibody pairDes-Serpro-Bnp antibody pairProbnp(79-108 - Bnp(3-30 - Bnp(3-29 - Brain Natriuretic Peptide 29 antibody pairBnp(4-32 - Bnp(4-31 - Bnp(4-30 - Bnp(4-29 - Bnp(4-27 - Bnp(5-32 - Bnp(5-31 - Bnp(5-29 antibody pairNPPB antibody pair |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
