| Host: | E.coli |
| Applications: | Enzyme Activity/SDS-PAGE |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human Biliverdin Reductase A/BLVRA-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications. |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin: | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity: | Specific activity is > 450pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of biliverdin of NADPH per minute at pH 8.8 at 25C. |
| Gene Symbol: | BLVRA |
| Gene ID: | 644 |
| Uniprot ID: | BIEA_HUMAN |
| Immunogen Region: | 3-296aa |
| Accession Number: | NP_000703 |
| Immunogen: | Human |
| Immunogen Sequence: | MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYL |
| Tissue Specificity | Liver. |
| Function | Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IXalpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. Does not reduce bilirubin IXbeta. Uses the reactants NADH or NADPH depending on the pH.NADH is used at the acidic pH range (6-6.9) and NADPH at the alkaline range (8.5-8.7). NADPH, however, is the probable reactant in biological systems. |
| Protein Name | Biliverdin Reductase ABvr ABiliverdin-Ix Alpha-Reductase |
| Database Links | Reactome: R-HSA-189483Reactome: R-HSA-9707564 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Protein Names | Biliverdin Reductase A proteinBvr A proteinBiliverdin-Ix Alpha-Reductase proteinBLVRA proteinBLVR proteinBVR protein |
Information sourced from Uniprot.org
