| Short Description : | Recombinant-Human Beta-NGF/NGF/NGFB-C-His protein was developed in hek293 cells using the region Glu19-Ala241. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Recombinant Human NGF stimulates cell proliferation of the TF‑1 human erythroleukemic cells. The ED50 for this effect is 2.2-8.6 ng/mL, corresponding to a specific activity of 1.16×105-4.55×105 units/mg. |
| Determination Method: | <0.01 EU/Mu g |
| Gene Symbol: | NGF |
| Gene ID: | 4803 |
| Uniprot ID: | NGF_HUMAN |
| Immunogen: | Recombinant Human Beta-NGF/NGF/NGFB Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu19-Ala241) of human NGF (Accession #NP_002497.2) fused with and a 6×His tag at the C-terminus. |
| Immunogen Region: | Glu19-Ala241 |
| Immunogen Sequence: | EPHSESNVPAGHTIPQAHWT KLQHSLDTALRRARSAPAAA IAARVAGQTRNITVDPRLFK KRRLRSPRVLFSTQPPREAA DTQDLDFEVGGAAPFNRTHR SKRSSSHPIFHRGEFSVCDS VSVWVGDKTTATDIKGKEVM VLGEVNINNSVFKQYFFETK CRDPNPVDSGCRGIDSKHWN SYCTTTHTFVKALTMDGKQA AWRFIRIDTACVCVLSRKAV RRA |
Information sourced from Uniprot.org
