Human Bcl-2 (Apoptosis Regulator Bcl-2) Antibody Pair (STJAP00467)
General Information
| Short Description | This Bcl-2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | BCL2 |
| Gene ID | 596 |
| Uniprot ID | BCL2_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human Bcl-2 protien.Detection Immunogen: Recombinant Human Bcl-2 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, which stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PRKN, leading to an increase in its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. Ubiquitinated by XIAP, leading to its degradation by the proteasome. |
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. |
| Protein Name | Apoptosis Regulator Bcl-2 |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-111453Reactome: R-HSA-6785807Reactome: R-HSA-844455Reactome: R-HSA-9018519Reactome: R-HSA-9634638Reactome: R-HSA-9818030Reactome: R-HSA-9824594 |
| Cellular Localisation | Mitochondrion Outer MembraneSingle-Pass Membrane ProteinNucleus MembraneEndoplasmic Reticulum MembraneCytoplasm |
| Alternative Antibody Pair Names | Apoptosis Regulator Bcl-2 antibody pairBCL2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Bcl-2 (Apoptosis Regulator Bcl-2) Antibody Pair (STJAP00467)
This antibody pair detects human Bcl-2 (Apoptosis Regulator Bcl-2, UniProt P10415) and is validated for human samples only. No cross-reactivity data for other species is provided.
Both antibodies are rabbit-derived and purified by antigen affinity. The capture antibody is unconjugated, supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol (pH 7.5), while the detection antibody is biotinylated, supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol (pH 7.5).
This pair is validated for use in ELISA. No other assay formats are listed in the product specification.
The listed compatible sample types are serum, plasma, and other biological fluids. These are all for in vitro research use only and should not be used for diagnostic or therapeutic purposes.
Both the capture and detection antibodies were raised against recombinant human Bcl-2 protein. No specific immunogen region is defined in the current product specification.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.