• Biotinylated Human BAFF (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human BAFF (Trimer) is greater than 95% as determined by SEC-HPLC.
  • Immobilized Human BAFFR, hFc Tag at 5 Mu g/ml (100 Mu l/Well) on the plate. Dose response curve for Biotinylated Human BAFF (Trimer) , His Tag with the EC50 of 6. 9ng/ml determined by ELISA.

Human BAFF/TNFSF13B/CD257 Trimer protein (Recombinant) (N-His-Avi) {Biotin} (STJP001665)

SKU:
STJP001665

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human BAFF/TNFSF13B/CD257 Trimer-N-His-Avi protein was developed from hek293 and has a target region of N-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: TNFSF13B
Gene ID: 10673
Uniprot ID: TN13B_HUMAN
Immunogen Region: Thr141-Leu285
Immunogen: DNA sequence encoding Human BAFF/TNFSF13B/CD257 Trimer including a N-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications The soluble form derives from the membrane form by proteolytic processing. Isoform 2 is not efficiently shed from the membrane unlike isoform 1. N-glycosylated.
Function Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3: Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.
Protein Name Tumor Necrosis Factor Ligand Superfamily Member 13b
B Lymphocyte Stimulator
Blys
B-Cell-Activating Factor
Baff
Dendritic Cell-Derived Tnf-Like Molecule
Tnf- And Apol-Related Leukocyte Expressed Ligand 1
Tall-1
Cd Antigen Cd257 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 13b - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 13b - Soluble Form
Database Links Reactome: R-HSA-5668541
Reactome: R-HSA-5669034
Reactome: R-HSA-5676594
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Tumor Necrosis Factor Ligand Superfamily Member 13b
Soluble Form: Secreted
Alternative Protein Names Tumor Necrosis Factor Ligand Superfamily Member 13b protein
B Lymphocyte Stimulator protein
Blys protein
B-Cell-Activating Factor protein
Baff protein
Dendritic Cell-Derived Tnf-Like Molecule protein
Tnf- And Apol-Related Leukocyte Expressed Ligand 1 protein
Tall-1 protein
Cd Antigen Cd257 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 13b - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 13b - Soluble Form protein
TNFSF13B protein
BAFF protein
BLYS protein
TALL1 protein
TNFSF20 protein
ZTNF4 protein
UNQ401 protein
PRO738 protein

Information sourced from Uniprot.org

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