| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | CD86 |
| Gene ID: | 942 |
| Uniprot ID: | CD86_HUMAN |
| Immunogen Region: | Leu26-Pro247 |
| Immunogen: | Recombinant Human B7-2/CD86 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu26-Pro247) of Human B7-2/CD86 (Accession #P42081-1) fused with a C-hFc tag at the C-terminus. |
| Immunogen Sequence: | LKIQAYFNETADLPCQFANS QNQSLSELVVFWQDQENLVL NEVYLGKEKFDSVHSKYMGR TSFDSDSWTLRLHNLQIKDK GLYQCIIHHKKPTGMIRIHQ MNSELSVLANFSQPEIVPIS NITENVYINLTCSSIHGYPE PKKMSVLLRTKNSTIEYDGV MQKSQDNVTELYDVSISLSV SFPDVTSNMTIFCILETDKT RLLSSPFSIELEDPQPPPDH IP |
| Tissue Specificity | Expressed by activated B-lymphocytes and monocytes. |
| Post Translational Modifications | Polyubiquitinated.which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. |
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. Isoform 2: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B. |
| Protein Name | T-Lymphocyte Activation Antigen Cd86Activation B7-2 AntigenB70Bu63Ctla-4 Counter-Receptor B7.2Fun-1Cd Antigen Cd86 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-2219530Reactome: R-HSA-389356Reactome: R-HSA-389357Reactome: R-HSA-389359Reactome: R-HSA-389513Reactome: R-HSA-6783783Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | T-Lymphocyte Activation Antigen Cd86 proteinActivation B7-2 Antigen proteinB70 proteinBu63 proteinCtla-4 Counter-Receptor B7.2 proteinFun-1 proteinCd Antigen Cd86 proteinCD86 proteinCD28LG2 protein |
Information sourced from Uniprot.org
