Human ATG4B protein (Recombinant) (N-His) (STJP016368)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ATG4B-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for further information. |
Target Information
| Gene Symbol | ATG4B |
| Gene ID | 23192 |
| Uniprot ID | ATG4B_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Leu393 |
Additional Info
| Function | Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. Required for canonical autophagy (macroautophagy), non-canonical autophagy as well as for mitophagy. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, by catalyzing delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Compared to other members of the family (ATG4A, ATG4C or ATG4C), constitutes the major protein for proteolytic activation of ATG8 proteins, while it displays weaker delipidation activity than other ATG4 paralogs. Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins: acts by regulating ATG9A trafficking to mitochondria and promoting phagophore-endoplasmic reticulum contacts during the lipid transfer phase of mitophagy. |
| Protein Name | Cysteine Protease Atg4bAut-Like 1 Cysteine EndopeptidaseAutophagy-Related Cysteine Endopeptidase 1Autophagin-1Autophagy-Related Protein 4 Homolog BHsapg4bHapg4b |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | CytoplasmCytosolCytoplasmic VesicleAutophagosomeEndoplasmic ReticulumMitochondrionMainly Localizes To The CytoplasmIncluding CytosolA Samll Potion Localizes To MitochondriaPhosphorylation At Ser-34 Promotes Localization To Mitochondria |
| Alternative Protein Names | Cysteine Protease Atg4b proteinAut-Like 1 Cysteine Endopeptidase proteinAutophagy-Related Cysteine Endopeptidase 1 proteinAutophagin-1 proteinAutophagy-Related Protein 4 Homolog B proteinHsapg4b proteinHapg4b proteinATG4B proteinAPG4B proteinAUTL1 proteinKIAA0943 protein |
Information sourced from Uniprot.org