| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This AQP3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
AQP3 |
| Gene ID: |
360 |
| Uniprot ID: |
AQP3_HUMAN |
| Specificity: |
This kit recognizes Human AQP-3 in samples. No significant cross-reactivity or interference between Human AQP-3 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Aquaglyceroporins form homotetrameric transmembrane channels, with each monomer independently mediating glycerol and water transport across the plasma membrane along their osmotic gradient. Could also be permeable to urea. Also participates in cell permeability to H2O2 and H2O2-mediated signaling. In skin, transports glycerol to the epidermis and stratum corneum, where it maintains hydration, elasticity, and supports lipid biosynthesis for barrier repair. In kidney, contributes to the reabsorption of water, helping the body maintain proper fluid balance. |
| Protein Name | Aquaporin-3Aqp-3Aquaglyceroporin-3 |
| Database Links | Reactome: R-HSA-432040Reactome: R-HSA-432047Reactome: R-HSA-9725554 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell Membrane |
| Alternative ELISA Names | Aquaporin-3 ELISA kitAqp-3 ELISA kitAquaglyceroporin-3 ELISA kitAQP3 ELISA kit |
| Specificity | This kit recognizes Human AQP-3 in samples. No significant cross-reactivity or interference between Human AQP-3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-109 | 100 |
| EDTA plasma (n=8) | 93-106 | 100 |
| Cell culture media (n=8) | 85-97 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.06 | 2.77 | 7.89 | 1.12 | 2.59 | 7.86 |
| Standard deviation | 0.06 | 0.14 | 0.38 | 0.06 | 0.15 | 0.32 |
| CV (%) | 5.66 | 5.05 | 4.82 | 5.36 | 5.79 | 4.07 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
