| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 37.50pg/mL |
| Detection Limit: | 62.50~4000ng/mL |
| Short Description : | This APOB Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, urine and saliva. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | APOB |
| Gene ID: | 338 |
| Uniprot ID: | APOB_HUMAN |
| Specificity: | This kit recognizes Human ApoB100 in samples. No significant cross-reactivity or interference between Human ApoB100 and analogues was observed. |
| Sample Type: | Serum, Plasma, Urine and Saliva |
| Function | Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. |
| Protein Name | Apolipoprotein B-100Apo B-100 Cleaved Into - Apolipoprotein B-48Apo B-48 |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-3000471Reactome: R-HSA-3000480Reactome: R-HSA-3000484Reactome: R-HSA-3000497Reactome: R-HSA-381426Reactome: R-HSA-432142Reactome: R-HSA-5686938Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8866423Reactome: R-HSA-8957275Reactome: R-HSA-8963888Reactome: R-HSA-8963901Reactome: R-HSA-8964026Reactome: R-HSA-8964038Reactome: R-HSA-8964041Reactome: R-HSA-8964046Reactome: R-HSA-9707616Reactome: R-HSA-975634 |
| Cellular Localisation | CytoplasmSecretedLipid Droplet |
| Alternative ELISA Names | Apolipoprotein B-100 ELISA kitApo B-100 Cleaved Into - Apolipoprotein B-48 ELISA kitApo B-48 ELISA kitAPOB ELISA kit |
| Specificity | This kit recognizes Human ApoB100 in samples. No significant cross-reactivity or interference between Human ApoB100 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-100 | 92 |
| EDTA plasma (n=8) | 84-98 | 90 |
| Cell culture media (n=8) | 87-100 | 92 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 188.30 | 533.30 | 1428.90 | 176.20 | 522.60 | 1444.00 |
| Standard deviation | 10.70 | 29.90 | 60.00 | 9.30 | 29.30 | 47.70 |
| CV (%) | 5.68 | 5.61 | 4.20 | 5.28 | 5.61 | 3.30 |
