Human ANP32A protein (Recombinant) (His-Tag) (STJP016852)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP016852
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General Information

Short DescriptionRecombinant-Human ANP32A-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 1mM DTT, 100mM NaCl, 0.1mM PMSF
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolANP32A
Gene ID8125
Uniprot IDAN32A_HUMAN
Accession NumberNP_006296
ImmunogenHuman
Immunogen Region1-249aa
Immunogen SequenceMGSSHHHHHH SSGLVPRGSH MEMGRRIHLE LRNRTPSDVK ELVLDNSRSN EGKLEGLTDE FEELEFLSTI NVGLTSIANL PKLNKLKKLE LSDNRVSGGL EVLAEKCPNL THLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLND YRENVFKLLP QLTYLDGYDR DDKEAPDSDA EGYVEGLDDE EEDEDEEEYD EDAQVVEDEE DEDEEEEGEE EDVSGEEE

Additional Info

Tissue Specificity Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
Post Translational Modifications Phosphorylated on serine residues, at least in part by casein kinase 2/CK2. The N-terminus is blocked. Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
Function Multifunctional protein that is involved in the regulation of many processes including tumor suppression, apoptosis, cell cycle progression or transcription. Promotes apoptosis by favouring the activation of caspase-9/CASP9 and allowing apoptosome formation. In addition, plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex. Inhibits the histone-acetyltranferase activity of EP300/CREBBP (CREB-binding protein) and EP300/CREBBP-associated factor by histone masking. Preferentially binds to unmodified histone H3 and sterically inhibiting its acetylation and phosphorylation leading to cell growth inhibition. Participates in other biochemical processes such as regulation of mRNA nuclear-to-cytoplasmic translocation and stability by its association with ELAVL1 (Hu-antigen R). Plays a role in E4F1-mediated transcriptional repression as well as inhibition of protein phosphatase 2A. (Microbial infection) Plays an essential role in influenza A, B and C viral genome replication. Mechanistically, mediates the assembly of the viral replicase asymmetric dimers composed of PB1, PB2 and PA via its N-terminal region. Also plays an essential role in foamy virus mRNA export from the nucleus.
Protein Name Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member A
Acidic Nuclear Phosphoprotein Pp32
Pp32
Leucine-Rich Acidic Nuclear Protein
Lanp
Mapmodulin
Potent Heat-Stable Protein Phosphatase 2a Inhibitor I1pp2a
Putative Hla-Dr-Associated Protein I
Phapi
Database Links Reactome: R-HSA-450520
Cellular Localisation Nucleus
Cytoplasm
Endoplasmic Reticulum
Translocates To The Cytoplasm During The Process Of Neuritogenesis
Shuttles Between Nucleus And Cytoplasm
Alternative Protein Names Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member A protein
Acidic Nuclear Phosphoprotein Pp32 protein
Pp32 protein
Leucine-Rich Acidic Nuclear Protein protein
Lanp protein
Mapmodulin protein
Potent Heat-Stable Protein Phosphatase 2a Inhibitor I1pp2a protein
Putative Hla-Dr-Associated Protein I protein
Phapi protein
ANP32A protein
C15orf1 protein
LANP protein
MAPM protein
PHAP1 protein

Information sourced from Uniprot.org

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