Human AMD1 protein (Recombinant) (His-Tag) (STJP017715)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP017715
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General Information

Short DescriptionRecombinant-Human AMD1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 20% Glycerol, 0.1M NaCl, 1mM DTT
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolAMD1
Gene ID262
Uniprot IDDCAM_HUMAN
Accession NumberNP_001625
ImmunogenHuman
Immunogen Region68-334aa
Immunogen SequenceSSMFV SKRRFILKTC GTTLLLKALV PLLKLARDYS GFDSIQSFFY SRKNFMKPSH QGYPHRNFQE EIEFLNAIFP NGAAYCMGRM NSDCWYLYTL DFPESRVISQ PDQTLEILMS ELDPAVMDQF YMKDGVTAKD VTRESGIRDL IPGSVIDATM FNPCGYSMNG MKSDGTYWTI HITPEPEFSY VSFETNLSQT SYDDLI

Additional Info

Post Translational Modifications Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.
Function Essential for biosynthesis of the polyamines spermidine and spermine. Promotes maintenance and self-renewal of embryonic stem cells, by maintaining spermine levels.
Protein Name S-Adenosylmethionine Decarboxylase Proenzyme
Adometdc
Samdc Cleaved Into - S-Adenosylmethionine Decarboxylase Alpha Chain - S-Adenosylmethionine Decarboxylase Beta Chain
Database Links Reactome: R-HSA-351202
Cellular Localisation
Alternative Protein Names S-Adenosylmethionine Decarboxylase Proenzyme protein
Adometdc protein
Samdc Cleaved Into - S-Adenosylmethionine Decarboxylase Alpha Chain - S-Adenosylmethionine Decarboxylase Beta Chain protein
AMD1 protein
AMD protein

Information sourced from Uniprot.org

Citations

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