Human Alpha 1-AT (Alpha-1-Antitrypsin) Antibody Pair (STJAP00233)

SPECIFICATIONS
ClonalityMonoclonal/Monoclonal
HostRabbit/Rabbit
ConjugationUnconjugated/Biotin
ImmunogenCapture immunogen: Recombinant Human Alpha 1-AT protein.
Detection Immunogen: Recombinant Human Alpha 1-AT protein.
STJAP00233-50
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General Information

Short DescriptionThis Alpha 1-AT antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA.
ApplicationsELISA
HostRabbit/Rabbit
ReactivityHuman
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

ClonalityMonoclonal/Monoclonal
ConjugationUnconjugated/Biotin
Concentration0.5 mg/mL
PurificationCapture Purification: Protein A.
Detection Purification: Protein A.
FormulationCapture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.
Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.
Storage InstructionStore at-20°C for the long term, and avoid freeze-thaw cycles.

Target Information

Gene SymbolSERPINA1
Gene ID5265
Uniprot IDA1AT_HUMAN
ImmunogenCapture immunogen: Recombinant Human Alpha 1-AT protein.
Detection Immunogen: Recombinant Human Alpha 1-AT protein.

Additional Info

Post Translational Modifications N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)Man(alpha1-3)Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases.
Function Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Protein Name Alpha-1-Antitrypsin
Alpha-1 Protease Inhibitor
Alpha-1-Antiproteinase
Serpin A1 Cleaved Into - Short Peptide From Aat
Spaat
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-204005
Reactome: R-HSA-381426
Reactome: R-HSA-5694530
Reactome: R-HSA-6798695
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Endoplasmic Reticulum
The S And Z Allele Are Not Secreted Effectively And Accumulate Intracellularly In The Endoplasmic Reticulum
Short Peptide From Aat: Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Pair Names Alpha-1-Antitrypsin antibody pair
Alpha-1 Protease Inhibitor antibody pair
Alpha-1-Antiproteinase antibody pair
Serpin A1 Cleaved Into - Short Peptide From Aat antibody pair
Spaat antibody pair
SERPINA1 antibody pair
AAT antibody pair
PI antibody pair
PRO0684 antibody pair
PRO2209 antibody pair

Information sourced from Uniprot.org

Citations

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