Human ALOX5 protein (Recombinant) (N-His) (STJP003917)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ALOX5-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | ALOX5 |
| Gene ID | 240 |
| Uniprot ID | LOX5_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Glu461-Ser671 |
Additional Info
| Function | Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation. Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene. Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses. Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers. In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes. Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40. May also play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK. Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells. |
| Protein Name | Polyunsaturated Fatty Acid 5-LipoxygenaseArachidonate 5-Lipoxygenase5-Lo5-Lipoxygenase |
| Database Links | Reactome: R-HSA-2142688Reactome: R-HSA-2142691Reactome: R-HSA-2142700Reactome: R-HSA-6785807Reactome: R-HSA-6798695Reactome: R-HSA-9012546Reactome: R-HSA-9018676Reactome: R-HSA-9018682Reactome: R-HSA-9018896Reactome: R-HSA-9020265Reactome: R-HSA-9023661Reactome: R-HSA-9026286Reactome: R-HSA-9026290Reactome: R-HSA-9026403Reactome: R-HSA-9027604 |
| Cellular Localisation | CytoplasmNucleus MatrixNucleus MembranePeripheral Membrane ProteinPerinuclear RegionCytosolNucleus EnvelopeNucleus Intermembrane SpaceShuttles Between Cytoplasm And NucleusFound Exclusively In The NucleusWhen Phosphorylated On Ser-272Calcium Binding Promotes Translocation From The Cytosol And The Nuclear Matrix To The Nuclear Envelope And Membrane Association |
| Alternative Protein Names | Polyunsaturated Fatty Acid 5-Lipoxygenase proteinArachidonate 5-Lipoxygenase protein5-Lo protein5-Lipoxygenase proteinALOX5 proteinLOG5 protein |
Information sourced from Uniprot.org