Human ALOX15B protein (Recombinant) (N-His) (STJP003233)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ALOX15B-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | ALOX15B |
| Gene ID | 247 |
| Uniprot ID | LX15B_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Arg253-Glu490 |
Additional Info
| Function | Isoform A: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist. Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates. Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively down-regulate the immune response and have anti-aggregation properties with platelets. In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids. Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells. Isoform B: Does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. |
| Protein Name | Polyunsaturated Fatty Acid Lipoxygenase Alox15b15-Lipoxygenase 215-Lox-2Arachidonate 15-Lipoxygenase B15-Lox-BArachidonate 15-Lipoxygenase Type IiLinoleate 13-Lipoxygenase 15-Lob |
| Database Links | Reactome: R-HSA-2142770 |
| Cellular Localisation | Isoform A: NucleusOther Isoforms Are Excluded From The NucleusCytoplasmCytosolCell MembraneCytoskeletonMembranePeripheral Membrane ProteinCell JunctionAdherens JunctionFocal AdhesionPredominantly CytosolicBecomes Enriched At Membranes Upon Calcium Binding |
| Alternative Protein Names | Polyunsaturated Fatty Acid Lipoxygenase Alox15b protein15-Lipoxygenase 2 protein15-Lox-2 proteinArachidonate 15-Lipoxygenase B protein15-Lox-B proteinArachidonate 15-Lipoxygenase Type Ii proteinLinoleate 13-Lipoxygenase 15-Lob proteinALOX15B protein |
Information sourced from Uniprot.org