| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 37.5pg/mL |
| Detection Limit: | 62.5~4000pg/mL |
| Short Description : | This human ADRA1A kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | ADRA1A |
| Gene ID: | 148 |
| Uniprot ID: | ADA1A_HUMAN |
| Specificity: | This kit recognizes Human ADRA1A in samples. No significant cross-reactivity or interference between Human ADRA1A and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. |
| Post Translational Modifications | C-terminal Ser or Thr residues may be phosphorylated. |
| Function | This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. |
| Protein Name | Alpha-1a Adrenergic Receptor Alpha-1a Adrenoreceptor Alpha-1a Adrenoceptor Alpha-1c Adrenergic Receptor Alpha-Adrenergic Receptor 1c |
| Database Links | Reactome: R-HSA-390696 Reactome: R-HSA-416476 Reactome: R-HSA-416482 |
| Cellular Localisation | Nucleus Membrane Multi-Pass Membrane Protein Cell Membrane Cytoplasm Membrane Caveola Location At The Nuclear Membrane Facilitates Heterooligomerization And Regulates Erk-Mediated Signaling In Cardiac Myocytes Colocalizes With Gnaq Plcb1 As Well As Lap2 At The Nuclear Membrane Of Cardiac Myocytes |
| Alternative CLIA Names | Alpha-1a Adrenergic Receptor CLIA kit Alpha-1a Adrenoreceptor CLIA kit Alpha-1a Adrenoceptor CLIA kit Alpha-1c Adrenergic Receptor CLIA kit Alpha-Adrenergic Receptor 1c CLIA kit ADRA1A CLIA kit ADRA1C CLIA kit |
| Specificity | This kit recognizes Human ADRA1A in samples. No significant cross-reactivity or interference between Human ADRA1A and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 98-111 | 105 |
| EDTA plasma(n=8) | 88-100 | 95 |
| Cell culture media(n=8) | 98-114 | 105 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 185.44 | 371.17 | 1994.50 | 194.54 | 337.46 | 2101.72 |
| Standard deviation | 16.58 | 33.52 | 220.39 | 19.42 | 30.24 | 217.74 |
| CV (%) | 8.94 | 9.03 | 11.05 | 9.98 | 8.96 | 10.36 |