Human ADAMTS4 (A disintegrin and metalloproteinase with thrombospondin motifs 4) CLIA Kit for Quantitative Detection

Name: Human ADAMTS4 (A disintegrin and metalloproteinase with thrombospondin motifs 4) CLIA Kit for Quantitative Detection
SKU: STJC0000172
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJC0000172

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General Information

Short Description	This human ADAMTS4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Target Information

Gene Symbol	ADAMTS4
Gene ID	9507
Uniprot ID	ATS4_HUMAN
Specificity	This kit recognizes Human ADAMTS4 in samples. No significant cross-reactivity or interference between Human ADAMTS4 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Tissue Specificity	Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Isoform 2: Detected in osteoarthritic synovium.
Post Translational Modifications	The precursor is cleaved by a furin endopeptidase. Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Can also be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion.
Function	Cleaves aggrecan, a cartilage proteoglycan, at the '392-Glu-\|-Ala-393' site and may be involved in its turnover. Also cleaves COMP. May play an important role in the destruction of aggrecan in arthritic diseases. Could be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease.
Protein Name	A Disintegrin And Metalloproteinase With Thrombospondin Motifs 4 Adam-Ts 4 Adam-Ts4 Adamts-4 Admp-1 Aggrecanase-1
Database Links	Reactome: R-HSA-1474228 Reactome: R-HSA-5083635 Reactome: R-HSA-5173214
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative CLIA Names	A Disintegrin And Metalloproteinase With Thrombospondin Motifs 4 CLIA kit Adam-Ts 4 CLIA kit Adam-Ts4 CLIA kit Adamts-4 CLIA kit Admp-1 CLIA kit Aggrecanase-1 CLIA kit ADAMTS4 CLIA kit KIAA0688 CLIA kit UNQ769 CLIA kit PRO1563 CLIA kit
Specificity	This kit recognizes Human ADAMTS4 in samples. No significant cross-reactivity or interference between Human ADAMTS4 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	97-114	105
EDTA plasma(n=8)	87-103	94
Cell culture media(n=8)	90-104	95

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	47.56	133.21	498.45	44.76	134.87	472.87
Standard deviation	4.96	10.14	51.64	4.39	15.47	52.49
CV (%)	10.43	7.61	10.36	9.81	11.47	11.10

Citations

View product citations for antibody STJC0000172 on CiteAb