Human ADAM15/MDC15 protein (Recombinant-Active) (C-10*His) (STJP012098)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human ADAM15/MDC15-C-10*His protein was developed from mammalian cells and has a target region of C-10*His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its ability of the Immobilised protein to support the adhesion of human acute T cell leukemia cells (Jurkat). The ED50 for this effect is 0.5-3 Mu g/mL. |
Target Information
| Gene Symbol | ADAM15 |
| Gene ID | 8751 |
| Uniprot ID | ADA15_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp207-Ser693 |
Additional Info
| Post Translational Modifications | The precursor is cleaved by a furin endopeptidase. Phosphorylation increases association with PTKs. |
| Function | Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. |
| Protein Name | Disintegrin And Metalloproteinase Domain-Containing Protein 15Adam 15Metalloprotease Rgd Disintegrin ProteinMetalloproteinase-Like - Disintegrin-Like - And Cysteine-Rich Protein 15Mdc-15Metargidin |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-8941237 |
| Cellular Localisation | Endomembrane SystemSingle-Pass Type I Membrane ProteinCell JunctionAdherens JunctionCell ProjectionCiliumFlagellumCytoplasmic VesicleSecretory VesicleAcrosomeThe Majority Of The Protein Is Localized In A Perinuclear Compartment Which May Correspond To The Trans-Golgi Network Or The Late EndosomeThe Pro-Protein Is The Major Detectable Form On The Cell SurfaceWhereas The Majority Of The Protein In The Cell Is Processed |
| Alternative Protein Names | Disintegrin And Metalloproteinase Domain-Containing Protein 15 proteinAdam 15 proteinMetalloprotease Rgd Disintegrin Protein proteinMetalloproteinase-Like - Disintegrin-Like - And Cysteine-Rich Protein 15 proteinMdc-15 proteinMetargidin proteinADAM15 proteinMDC15 protein |
Information sourced from Uniprot.org