Human ACTG2 protein (Recombinant) (N-His) (STJP005050)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ACTG2-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | ACTG2 |
| Gene ID | 72 |
| Uniprot ID | ACTH_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Glu3-Phe376 |
Additional Info
| Post Translational Modifications | Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization. Actin, gamma-enteric smooth muscle, intermediate form: N-terminal cleavage of acetylated cysteine of intermediate muscle actin by ACTMAP. Monomethylation at Lys-85 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Methylated at His-74 by SETD3. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-51 of one monomer and Glu-271 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners. |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Protein Name | Actin - Gamma-Enteric Smooth MuscleAlpha-Actin-3Gamma-2-ActinSmooth Muscle Gamma-Actin Cleaved Into - Actin - Gamma-Enteric Smooth Muscle - Intermediate Form |
| Database Links | Reactome: R-HSA-445355Reactome: R-HSA-9913351 |
| Cellular Localisation | CytoplasmCytoskeleton |
| Alternative Protein Names | Actin - Gamma-Enteric Smooth Muscle proteinAlpha-Actin-3 proteinGamma-2-Actin proteinSmooth Muscle Gamma-Actin Cleaved Into - Actin - Gamma-Enteric Smooth Muscle - Intermediate Form proteinACTG2 proteinACTA3 proteinACTL3 proteinACTSG protein |
Information sourced from Uniprot.org