| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. or Supplied as a 0.22 Mu m filtered solution in PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1.0 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 at 2 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Spike RBD with a linear range of 0.03-4.9 ng/mL. |
| Gene Symbol: | ACE2 |
| Gene ID: | 59272 |
| Uniprot ID: | ACE2_HUMAN |
| Immunogen Region: | Gln18-Ser740 |
| Immunogen: | Active Recombinant Human ACE2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession #Q9BYF1) fused with a 6×His, Avi tag at the C-terminus. |
| Immunogen Sequence: | QSTIEEQAKTFLDKFNHEAE DLFYQSSLASWNYNTNITEE NVQNMNNAGDKWSAFLKEQS TLAQMYPLQEIQNLTVKLQL QALQQNGSSVLSEDKSKRLN TILNTMSTIYSTGKVCNPDN PQECLLLEPGLNEIMANSLD YNERLWAWESWRSEVGKQLR PLYEEYVVLKNEMARANHYE DYGDYWRGDYEVNGVDGYDY SRGQLIEDVEHTFEEIKPLY EHLHAYVRAKLMNAYPSYI |
| Tissue Specificity | Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level). Expressed in enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in the renal proximal tubule and the small intestine (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system (at protein level). In lung, expressed at low levels in some alveolar type 2 cells, the expression seems to be individual-specific (at protein level). Expressed in nasal epithelial cells (at protein level). Coexpressed with TMPRSS2 within some lung alveolar type 2 cells, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells. Coexpressed with TMPRSS4 within mature enterocytes. Isoform 2: Expressed in nasal and bronchial epithelial cells (at protein level). |
| Post Translational Modifications | N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. Phosphorylated. Phosphorylation at Tyr-781 probably inhibits interaction with AP2M1 and enables interactions with proteins containing SH2 domains. Ubiquitinated. Ubiquitinated on Lys-788 via 'Lys-48'-linked ubiquitin. 'Lys-48'-linked deubiquitinated by USP50 on the Lys-788.leading to its stabilization. |
| Function | Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, Pyr1apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency. In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2: Non-functional as a carboxypeptidase. Isoform 2: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2. |
| Protein Name | Angiotensin-Converting Enzyme 2 Angiotensin-Converting Enzyme Homolog Aceh Angiotensin-Converting Enzyme-Related Carboxypeptidase Ace-Related Carboxypeptidase Metalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2 |
| Database Links | Reactome: R-HSA-2022377 Reactome: R-HSA-9678110 Reactome: R-HSA-9679191 Reactome: R-HSA-9694614 Reactome: R-HSA-9733458 |
| Cellular Localisation | Processed Angiotensin-Converting Enzyme 2: Secreted Cell Membrane Single-Pass Type I Membrane Protein Cytoplasm Cell Projection Cilium Apical Cell Membrane Detected In Both Cell Membrane And Cytoplasm In Neurons Isoform 2: Apical Cell Membrane |
| Alternative Protein Names | Angiotensin-Converting Enzyme 2 protein Angiotensin-Converting Enzyme Homolog protein Aceh protein Angiotensin-Converting Enzyme-Related Carboxypeptidase protein Ace-Related Carboxypeptidase protein Metalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2 protein ACE2 protein UNQ868 protein PRO1885 protein |
Information sourced from Uniprot.org
