Human ACAT1 protein (Recombinant) (His-Tag) (STJP017908)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP017908
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General Information

Short DescriptionRecombinant-Human ACAT1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration1 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% Glycerol, 1mM DTT
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolACAT1
Gene ID38
Uniprot IDTHIL_HUMAN
Accession NumberNP_000010
ImmunogenHuman
Immunogen Region34-427aa
Immunogen SequenceVSKPTLK EVVIVSATRT PIGSFLGSLS LLPATKLGSI AIQGAIEKAG IPKEEVKEAY MGNVLQGGEG QAPTRQAVLG AGLPISTPCT TINKVCASGM KAIMMASQSL MCGHQDVMVA GGMESMSNVP YVMNRGSTPY GGVKLEDLIV KDGLTDVYNK IHMGSCAENT AKKLNIARNE QDAYAINSYT RSKAAWEAGK FGNEVI

Additional Info

Post Translational Modifications Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5.
Function This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.
Protein Name Acetyl-Coa Acetyltransferase - Mitochondrial
Acetoacetyl-Coa Thiolase
T2
Database Links Reactome: R-HSA-70895
Reactome: R-HSA-77108
Reactome: R-HSA-77111
Reactome: R-HSA-9837999
Reactome: R-HSA-9854311
Reactome: R-HSA-9915355
Cellular Localisation Mitochondrion
Alternative Protein Names Acetyl-Coa Acetyltransferase - Mitochondrial protein
Acetoacetyl-Coa Thiolase protein
T2 protein
ACAT1 protein
ACAT protein
MAT protein

Information sourced from Uniprot.org

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